中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 响应面试验优化双水相萃取大吴风草总黄酮工艺及抑菌活性测定 食品科学 2015,36 (24) 57-62
Position: Home > Articles > Optimization of Aqueous Two-Phase Extraction of Total Flavonoids from Farfugium japonicum(L. f.) Kitam by Response Surface Methodology and Antibacterial Activity FOOD SCIENCE 2015,36 (24) 57-62

响应面试验优化双水相萃取大吴风草总黄酮工艺及抑菌活性测定

作  者：
陈建中;葛水莲;昝立峰;邢浩春;付静;王更先
单  位：
邯郸学院生命科学与工程学院
关键词：
响应面优化;双水相萃取;黄酮类物质;大吴风草;抑菌活性
摘  要：
目的:优化大吴风草总黄酮(total flavonoids of Farfugium,TFF)双水相萃取体系并研究其抑菌活性。方法:超声波辅助C2H5OH-(NH4)2SO4双水相萃取TFF,依据Box-Behnken试验设计原理,采用三因素三水平响应面分析法,以TFF萃取率为响应值进行方差分析,获取多元二次线性回归方程;采用K-B纸片扩散法测定6种供试菌种的抑菌圈直径,对比最小抑菌浓度(minimum inhibitory concentration,MIC)和最小杀菌浓度(minimum bactericidal concentration,MBC)确定其抑菌活性。结果:24%C2H5OH-18%(NH4)2SO4双水相萃取体系条件下TFF萃取率最高,响应面试验方差分析表明,粗提液质量分数显著影响TFF萃取率(P=0.023 9<0.05),而p H值和Na Cl质量分数对萃取率的影响不显著;最佳双水相萃取条件为粗提液质量分数20%、p H 7.64、Na Cl质量分数2.68%,萃取率模型预测最大值为96.366 7%(P=0.994);TFF对枯草芽孢杆菌抑制作用最强,高剂量作用下抑菌率可达98.67%,MIC为1.56 mg/m L;对沙门氏菌、金黄色葡萄球菌和大肠杆菌抑制作用次之,对青霉和黑曲霉抑制作用最弱。结论:粗提液质量分数对萃取率影响较大,p H值和Na Cl质量分数对萃取率的影响较小;TFF对6种供试菌种均表现出较强的抑菌活性,抑菌率与TFF质量浓度呈正相关,TFF对细菌的抑制效果相对强于真菌。
译  名：
Optimization of Aqueous Two-Phase Extraction of Total Flavonoids from Farfugium japonicum(L. f.) Kitam by Response Surface Methodology and Antibacterial Activity
作  者：
CHEN Jianzhong;GE Shuilian;ZAN Lifeng;XING Haochun;FU Jing;WANG Gengxian;College of Life Science and Engineering, Handan College;Wild Resources Plant Research Center of South Hebei Mt.Taihang;
关键词：
response surface methodology;;aqueous two-phase extraction;;total flavonoids;;F.japonicurn(L.f.) Kitam;;antimicrobial activity
摘  要：
Objective: To optimize the aqueous phase extraction system for total flavonoids(TFF) from the aboveground parts of Farfugium japonicum and to study the antibacterial activity of the extract. Methods: Ultrasonic was used to assist C2H5OH-(NH4)2SO4 aqueous two-phase extraction of TFF, and the multivariate quadratic regression model using TFF yield as the response variable was established based on a Box-Behnken experimental design involving three factors at three levels each and subjected to analysis of variance(ANOVA) and response surface analysis. The Kirby-Bauer disk diffusion method was used to determine the diameters of inhibition zone of six tested strains and the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) values were compared to study their antibacterial activities. Results: The 24% C2H5OH-18%(NH4)2SO4 aqueous two-phase extraction system was found to be optimal to extract TFF. The results of ANOVA showed that mass fraction of TFF in crude ethanolic extracts significantly affected the extraction rate of TFF(P = 0.023 9 < 0.05), but the effects of p H and Na Cl concentration in the aqueous two-phase system were not significant. The optimal extraction conditions were determined as follows: crude extract concentration, 20%; p H, 7.64; and Na Cl concentration, 2.68%. Under these conditions, the maximum extraction rate of 96.366 7%(P = 0.994) was obtained. The inhibitory effect of TFF on Bacillus subtilis was the strongest with a percentage inhibition of 98.67% at high dose and an MIC of 1.56 mg/m L, followed by Salmonella, Staphylococcus aureus and Escherichia coli, and the inhibitory effects on Penicillium and Aspergillus niger were the weakest. Conclusions: The flavonoid concentration of crude extracts had greater influence on the extraction rate of TFF than p H and Na Cl. TFF showed obvious antibacterial activity against the six tested strains. The percentage inhibition was positively correlated to TFF concentration. The antibacterial activity of TFF was relatively stronger against bacteria than fungi.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46