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当前位置: 首页 > 文章 > 条斑紫菜多糖对人肝癌Bel7402抗肿瘤作用的初步研究 中国食物与营养 2010,2010 (08) 82-84
Position: Home > Articles > Study on the Anti-tumor Effects of Porphyra Yezoensis Polysaccharide on Human Hepatocellular Carcinoma Bel7402 Cells Food and Nutrition in China 2010,2010 (08) 82-84

条斑紫菜多糖对人肝癌Bel7402抗肿瘤作用的初步研究

作  者：
张杰;杨旭东;王崴
单  位：
牡丹江医学院;哈尔滨市中医医院
关键词：
条斑紫菜多糖;肝癌;Bax基因;Survivin基因
摘  要：
目的:研究条斑紫菜多糖对人肝癌Bel7402体外抗肿瘤作用。方法:采用MTT法观察条斑紫菜多糖体外对肝癌Bel7402的增殖抑制作用;荧光显微镜观察不同浓度条斑紫菜多糖诱导Bel7402细胞凋亡作用情况,RT-PCR检测Survivin、Bax基因表达量的变化。结果:条斑紫菜多糖对人肝癌Bel7402细胞有显著地增殖抑制作用,能诱导Bel7402细胞凋亡,并且能上调Bax、降低Survivin表达。结论:条斑紫菜多糖体外有一定的抗肿瘤作用,并能促进Bel7402细胞凋亡,其机制可能与激活Bax、抑制Survivin表达有关。
译  名：
Study on the Anti-tumor Effects of Porphyra Yezoensis Polysaccharide on Human Hepatocellular Carcinoma Bel7402 Cells
作  者：
ZHANG Jie1,YANG Xu-dong1,WANG Wei2 (1 Mudanjiang Medical College, Mudanjiang 157011; 2 Harbin Chinese Medicine Hospital, Haerbin 150076)
关键词：
Porphyra yezoensis Polysaccharide;Hepatocellular Carcinoma;Bax Gene;Survivin Gene
摘  要：
【Objective】To observe the anti-tumor activity of Porphyra yezoensis polysaccharide on Bel7402 cells in vitro. 【Method】In vitro, the inhibitory effects of Porphyra yezoensis polysaccharide on proliferation of Bel7402 cells were measured by MTT colorimetric assay. Bel7402 cells were treated with various doses of Porphyra yezoensis polysaccharide. Apoptosis of Bel7402 cells were observed by fluorescence microscope. The levels of survivin and bax gene expression were detected by RT-PCR. 【Result】The studies demonstrated that Porphyra yezoensis polysaccharide inhibited proliferation of Bel7402 cells. There was obviously apoptosis-inducing effect on Bel7402 cells and it could effectively decrease the level of survivin gene expression and improve the level of bax gene expression. 【Conclusion】Porphyra yezoensis polysaccharide had anti-tumor effect on Bel7402 cells and could induce apoptosis of Bel7402 cells probably through activating survivin gene expression and inhibiting bax gene expression.

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