当前位置: 首页 > 文章 > 牛精子体外获能前后元素变化的研究 中国农业科学 1993,26 (2) 63-69+98
Position: Home > Articles > STUDIES ON CHANGES OF ELEMENT CONTENTS IN CATTLE SPERM OF PRE-CAPACITATION AND POST-CAPACITATION IN VITRO Scientia Agricultura Sinica 1993,26 (2) 63-69+98

牛精子体外获能前后元素变化的研究

作  者:
冯怀亮;杨庆章;房秀;秦鹏春;刘建民
单  位:
中国人民解放军兽医大学;东北农学院生物工程系
关键词:
牛精子;精子获能;元素;x射线微分析
摘  要:
本文应用x射线能谱技术,对获能前后精子质膜表面(头部、中段)和内部(顶体内、中段线粒体内)的元素进行了分析测试。结果表明,牛精子用肝素+咖啡因体外获能后,头部、中段质膜表面的Na、Al、S、K含量升高,Cl、Ca含量降低;顶体内Na、Mg、Si、P和Fe含量降低,而Ca、K、Cl和S含量升高;精子中段线粒体内Na、P、Si、Cl和Ca升高,而Mg、S、K和Fe却降低。获能过程中精子顶体内Na/Ca、Na/K、Cl/Ca、Cl/K和Na/Cl的浓度比分别为42.13、12.43、15.36、45.31和2.74;而摩尔比又分别为73.42、21.13、17.37、4.99和4.23。本文并对可能的获能机理进行了讨论。
译  名:
STUDIES ON CHANGES OF ELEMENT CONTENTS IN CATTLE SPERM OF PRE-CAPACITATION AND POST-CAPACITATION IN VITRO
作  者:
Feng Huailiang Yang Qingzhang Fang Xiu Qin Pengchun (Northeast Agricultural College, Harbin 150030) Liu Jianmin (Veterinary College of PLA, Changchun, 130062
关键词:
Cattle sperm; Element; Sperm capacitation; X-ray microanalysis
摘  要:
The surface elements and inner elements of pre-capacitation and post-capacitation were tested with scanning electron microscopy and transmission electron microscopy energy dispersive X-ray microanalysis technique. The results showed that Na, Al, S, and K elements increased, Cl and Ca elements decreased in plasma membrane surfaces of sperm head and midpiece with heparin-caffeine capacitation; Na, Mg, Si, P, and Fe elements decreased; Ca,K, Cl and S increased in sperm acrosome; Na, P, Si, Cl and Ca increased, Mg, S, K and Fe elements became lower in sperm midpiece mitochondria. During sperm capacitation, Na/Ca, Na/K, Cl/Ca, Cl/K and Na/Cl consistency ratios were 42.13, 12.43, 15.36, 45.31 and 2.74 respectively, also molar ratios were 73.42, 21.13, 17.37, 4.99 and 4.23 respectively. The probable capacitation mechanisim is discussed in this paper.

相似文章

计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊