中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 前体及营养物提高蛹虫草虫草菌素产量的研究 食品科学 2010,31 (5) 175-179
Position: Home > Articles > Enhanced Production of Cordycepin by Submerged Culture Using Additives in Cordyceps militaris FOOD SCIENCE 2010,31 (5) 175-179

前体及营养物提高蛹虫草虫草菌素产量的研究

作  者：
文庭池;康冀川;雷帮星;李光荣;何劲
单  位：
贵州大学贵州省生化工程中心
关键词：
蛹虫草;液体深层发酵;前体;优化;虫草菌素
摘  要：
研究不同前体及营养物对蛹虫草液体深层发酵产胞外虫草菌素的影响,并优化其促进虫草菌素生产的最佳添加条件。结果表明:添加腺嘌呤、甘氨酸、腺苷、L-谷氨酰胺4种前体或营养物能大幅提高蛹虫草液体发酵胞外虫草菌素的产量。两种前体和营养物能够通过协同互补作用提高胞外虫草菌素的产量,而且腺嘌呤的组合添加效果明显高于腺苷组合,特别是1g/L的腺嘌呤与8g/L的甘氨酸组合添加胞外虫草菌素产量达到1644.21mg/L,是基础培养基的4.66倍。不同前体及营养物添加发酵后其核苷类物质的含量表明:80%的虫草菌素被分泌到发酵液中,蛹虫草整个核苷酸代谢系统是紧密相连的,很多核苷类物质都能直接或间接转化成虫草菌素。
译  名：
Enhanced Production of Cordycepin by Submerged Culture Using Additives in Cordyceps militaris
作  者：
WEN Ting-chi1,2,KANG Ji-chuan1,2,LEI Bang-xing1,2,LI Guang-rong1,HE Jin1 (1. Biochemistry Engineering Research Center of Guizhou Province, Guizhou University, Guiyang 550025,China; 2. Engineering Research Center of Pharmaceutical Bio-resources of Southwest China, Ministry of Education, Guiyang 550025,China)
关键词：
Cordyceps militaris;submerged culture;additives;optimization;cordycepin
摘  要：
The effects of different additives on cordycepin production by submerged culture of Cordyceps militaris were investigated. Results indicated that the addition of adenine, glycine, adenosine or L-glutamine could result in a large improvement in cordycepin production by submerged fermentation of C. militaris. In addition, a synergistic effect on cordycepin production was also observed in combinatorial applications of two additives. Moreover, in the combinatorial application, adenine was much better than adenosine, especially the combinatorial formula of 1 g/L adenine and 8 g/L glycine, which resulted in a cordycepin production of 1644.21 mg/L, exhibiting a 4.66-fold increase than that obtained in the basal medium culture. The analysis of nucleic acid-related compounds obtained by various cultures indicated that 80% cordycepin synthesized by C. militaris was secreted in the culture broth. The whole metabolism system for nucleic acids were linked to each other so that most nucleic acidrelated compounds could directly or indirectly participate in the production of cordycepin.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46