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当前位置: 首页 > 文章 > 稀硫酸处理促进辐照芒草木质纤维素酶解糖化条件的优化 安徽农业科学 2013 (31) 12455-12457+12461
Position: Home > Articles > Optimization of Conditions on Dilute H_2SO_4 Treatment to Improve Enzyme Decomposition Saccharify of Lignocellulose from Chinese Silvergrass Treated by Irradiation Journal of Anhui Agricultural Sciences 2013 (31) 12455-12457+12461

稀硫酸处理促进辐照芒草木质纤维素酶解糖化条件的优化

作  者：
彭姿;谭兴和;熊兴耀;苏小军;张春艳;张涛;王克勤
单  位：
湖南生物机电职业技术学院;湖南农业大学食品科技学院;湖南省核农学与航天育种研究所;生物质醇类燃料湖南省工程实验室
关键词：
硫酸;芒草;辐照;木质纤维素;纤维素酶;糖化
摘  要：
[目的]考察稀硫酸对辐照芒草木质纤维素酶解糖化的促进作用。[方法]选取400 kGy60Coγ-射线辐照过的芒草,在不同浓度硫酸、处理时间及温度下对其进行水解,再用300 U/g纤维素酶处理96 h,测定还原糖含量。[结果]试验表明,经400 kGy60Coγ-射线辐照过的芒草在5%硫酸浓度下90℃水浴4 h,还原糖含量为60.33 mg/g;再将酸处理过的辐照芒草残渣用300 U/g的纤维素酶酶解,还原糖含量可达278.38 mg/g(以辐照芒草质量计算),两步处理总还原糖为338.71 mg/g,表明5%的硫酸可明显促进辐照芒草木质纤维素酶解糖化。[结论]研究可为辐照芒草木质纤维素酶解糖化工艺提供参考依据。
译  名：
Optimization of Conditions on Dilute H_2SO_4 Treatment to Improve Enzyme Decomposition Saccharify of Lignocellulose from Chinese Silvergrass Treated by Irradiation
作  者：
PENG Zi;College of Food Science and Technology,Hunan Agricultural University;
关键词：
H2SO4;;Chinese silvergrass;;Irradiation;;Lignocellulose;;Celluase;;Saccharify
摘  要：
[Objective] To investigate the promotion effect of dilute H2SO4on enzyme decomposition saccharify of lignocellulose from Chinese silvergrass treated by irradiation.[Method] This experiment selected Chinese silvergrass of 400 kGy60Coγ-ray irradiation,disposed it by 300 U/g celluase at 96 h,the reducing sugar content was determined.[Result]The results showed that the concentration of 5% H2SO4at 90 ℃ for 4 h,the reducing sugar production was 60.33 mg/g.Then collected the residue by H2SO4treatment to 300 U/g enzyme decomposition saccharify,the reducing sugar production was 278.38 mg/g(calculate it in Chinese silvergrass treated by irradition).The total reducing sugar production was 338.71 mg/g by these two steps,that was to say 5% H2SO4could improve enzyme decomposition saccharify of lignocellulose from Chinese silvergrass treated by irradiation.[Conclusion] The study can provide reference basis for enzyme decomposition saccharify of lignocellulose from Chinese silvergrass treated by irradiation.

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