中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 水果及其制品中果胶含量的比色法测定条件优化 果树学报 2012 (2) 302-307
Position: Home > Articles > Study on the colorimetry determination conditions of pectin in fruits and derived products Journal of Fruit Science 2012 (2) 302-307

水果及其制品中果胶含量的比色法测定条件优化

作  者：
庞荣丽;张巧莲;郭琳琳;方金豹;谢汉忠;李君;罗静;吴丰魁
单  位：
许昌职业技术学院园林园艺系;中国农业科学院郑州果树研究所/农业部果品及苗木质量监督检验测试中心(郑州)
关键词：
果胶;比色法;硫酸;咔唑
摘  要：
为研究水果及其制品中果胶测定方法,以半乳糖醛酸为标准物质,对硫酸-咔唑比色法测定水果及其制品中果胶含量的比色条件如吸收波长、咔唑-无水乙醇溶液用量、硫酸用量、显色温度、显色时间、显色后稳定时间等进行了优化和系统研究。结果表明,最佳显色条件为在1.0 mL滤液中,加入0.25 mL 0.1%咔唑-无水乙醇溶液,迅速加入5.0 mL硫酸,在85℃水浴中加热20 min,自来水浴中冷却,显色后1.5 h内在525 nm波长条件下测定。在此条件下,标准曲线线性范围广、相关性良好,精密度和准确度高,均符合相关标准要求。
译  名：
Study on the colorimetry determination conditions of pectin in fruits and derived products
作  者：
PANG Rong-li1,ZHANG Qiao-lian2,GUO Lin-lin1,FANG Jin-bao1,XIE Han-zhong1,LI Jun1,LUO Jing1,WU Feng-kui1(1Zhengzhou Fruit Research Institute,CAAS/ Monitor & Test Center of Fruit and Nursery Stock Quality,the Ministry of Agriculture,Zhengzhou,Henan 450009 China;2Department of Landscape & Horticulture,Xuchang Vocational Technical College,Xuchang,Henan 461000 China)
关键词：
Pectin;Colorimetry;Sulfuric acid;Carbazole
摘  要：
In order to research the determination method of pectin content in fruits and derived products,coloring conditions of sulfuric acid-carbazole colorimetry including absorption wavelength,amount of carbazole-ethanol solution and sulfuric acid,temperature,reaction time,and stabilization time after coloring were optimized using galacturonic acid as standard substance.The results showed that the optimum coloring conditions were adding 0.25 mL 0.1% carbazole-ethanol solution and adding 5.0 mL sulfuric acid quickly to 1.0 mL filtrate,water bathing for 20 min at 85 ℃,cooling down quickly with running tape water bath,and measuring at 525 nm wavelength within 1.5 h after coloring.Under the conditions,it had wide linear range standard curve,good correlation,high precision and accuracy,and were in line with relevant standards.

相似文章

导出引用
计量
文章访问数: 17
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46