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当前位置: 首页 > 文章 > 通过优化超排后合笼时间高效获取小鼠孵化囊胚 中国畜牧兽医 2015,42 (5) 1253-1258
Position: Home > Articles > Study on Time Period Model for Efficiently Collecting Hatched Blastocysts Flushed from Murine Uterus China Animal Husbandry & Veterinary Medicine 2015,42 (5) 1253-1258

通过优化超排后合笼时间高效获取小鼠孵化囊胚

作  者：
翟椿东;顾美超;刘迪;王力红;齐晓龙;张嘉楠;刘云海;倪和民;郭勇
单  位：
北京农学院动物科学技术学院
关键词：
小鼠;孵化囊胚;超数排卵;合笼时间
摘  要：
本试验旨在优化小鼠超排后的合笼时间,高效获取小鼠孵化囊胚。试验选用150只ICR系8周龄雌性小鼠,随机分为5组,同一时间超排处理后雌、雄按1∶1于18∶00、19∶00、20∶00、21∶00合笼过夜,次日上午08∶00查栓,发现阴道栓这为妊娠第1天(D1)。取妊娠第5天(D5)小鼠处死,剪取双侧子宫角,冲取胚胎。统计每组冲取胚胎的总数及孵化囊胚/未孵化囊胚的比值,作为胚胎获取效率的评价指标;统计内细胞团数/滋养外胚层细胞团数,作为评价胚胎质量的参考指标。结果发现,在数量上组Ⅰ、组Ⅱ、组Ⅲ囊胚数差异不显著(P>0.05),但有增高趋势,组Ⅳ囊胚数显著高于其他3组(P<0.05)。组Ⅰ、组Ⅱ、组Ⅲ、组Ⅳ内细胞团数/滋养外胚层细胞团数分别为23.18%、23.55%、21.72%和23.28%,各组间差异不显著(P>0.05)。结果表明,组Ⅳ所对应的合笼时间获取小鼠孵化囊胚获取效率最高,胚胎囊胚质量无明显差异。
译  名：
Study on Time Period Model for Efficiently Collecting Hatched Blastocysts Flushed from Murine Uterus
作  者：
ZHAI Chun-dong;GU Mei-chao;LIU Di;WANG Li-hong;QI Xiao-long;ZHANG Jia-nan;LIU Yun-hai;NI He-min;GUO Yong;College of Animal Science and Technology,Beijing University of Agriculture;
关键词：
mouse;;hatched blastocysts;;superovulation;;cage time
摘  要：
The persent study was aimed to investigate the optimization of mice cage time after superovulation,and efficient acquisition of mouse hatched blastocysts.The experiment selected 150 ICR female mice aged 8weeks,were randomly divided into 5groups,the same time superovulation treatment according to 1∶1,after the male and female in 18∶00,19∶00,20∶00,21∶00alloy cage overnight,the next day as early as 08∶00check,found that vaginal suppository for the first day of the pregnancy(D1).Take the pregnant the fifth day(D5)mice were sacrificed,their bilateral uterine horns,rushed from the embryonic.Statistics each thrust ratio and the total number of embryos hatched blastocysts/take not hatched blastocysts,was used as the index to evaluate embryos to obtain efficiency;Statistical trophectoderm cell number/inner cell mass number,as a reference index to evaluate the quality of embryo.The results found that groups of Ⅰ,Ⅱ and Ⅲwere no significant difference in the blastocyst number(P>0.05),but there was increasing trend,group Ⅳ was significantly higher than the other three groups(P<0.05).GroupsⅠ,Ⅱ,ⅢandⅣ within the cell mass cells number/trophectoderm cell number were 23.18%,23.55%,21.72% and 23.28%,there was no significant difference in each group(P>0.05).The results showed that the corresponding set ofⅣ cage got the time of mouse hatched blastocysts to obtainthe highest efficiency,there was no significant differences in embryonic blastocyst quality.

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