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Position: Home > Articles > Separation and Antioxidant Activity of Corn Gluten Meal Hydrolysate FOOD SCIENCE 2010,31 (1) 117-122

玉米蛋白粉水解产物的分离及其抗氧化活性

作  者:
李鸿梅;杨锐;于雷;张学忠
单  位:
吉林大学分子酶学与工程教育部重点实验室;吉林农业大学食品科学与工程学院
关键词:
玉米蛋白粉;分离纯化;清除自由基活性;抗氧化活性
摘  要:
以碱性蛋白酶做催化剂水解玉米蛋白粉,水解产物精制后,采用SephedaxG-15和G-10葡聚糖凝胶层析法及高效液相层析法进行分离纯化。以α-生育酚为阳性对照,研究水解产物及其分离组分对DPPH自由基的清除活性和对亚油酸自氧化的抑制作用,同时测定它们的还原力。采用邻苯三酚自氧化法研究它们对超氧阴离子自由基的清除作用。结果表明,抗氧化活性最高的组分是经SephedaxG-10层析法分离得到的组分Ⅱ。组分Ⅱ的分子量分布范围在400~700D之间,富含谷氨酸、丙氨酸、亮氨酸、组氨酸、脯氨酸和苯丙氨酸。
译  名:
Separation and Antioxidant Activity of Corn Gluten Meal Hydrolysate
作  者:
LI Hong-mei1,YANG Rui1,YU Lei1,ZHANG Xue-zhong2(1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China;2.Key Laboratory of Molecular Enzymology and Engineering,Ministry of Education,Jilin University,Changchun 130021,China)
关键词:
corn gluten meal;separation and purification;free radical scavenging activity;antioxidant activity
摘  要:
Corn gluten meal was hydrolyzed by proteolysis with alkaline protease and the refined hydrolysate was then separated and purified by Sephadex G-15 and G-10 column chromatographic and preparative HPLC methods.With α-tocopherol as a positive control,the hydrolysate and its fractions were evaluated for antioxidant activity by DPPH radical scavenging assay,linoleic acid auto-oxidation inhibition assay,ferric reducing/antioxidant power(FRAP) assay and superoxide anion scavenging assay based on pyrogallol auto-oxidation method.Results revealed that fraction II eluted from Sephadex G-10 column exhibited the highest antioxidant activity,a relative molecular mass distributed in range of 400 to 700 D detected by MS and an amino acid composition mainly consisting of glutamic acid,alanine,leucine,histidine,proline and phenylalanine.

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