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Position: Home > Articles > Fingerprint Analysis and Content Determination of Lipid Components in Ganoderma lucidum Spore Powder by HPLC-ELSD Acta Edulis Fungi 2023,30 (6) 52-59

灵芝孢子粉脂质成分HPLC-ELSD指纹图谱构建及含量测定

作  者:
夏凤娜;关小莺;陈少丹;陈秋颜;张一帆;杨小兵
单  位:
关键词:
灵芝孢子粉;高效液相色谱-蒸发光散射检测;脂质成分;指纹图谱
摘  要:
以16批(S1~S16)不同来源的灵芝(Ganoderma lucidum)孢子粉为材料,构建灵芝孢子粉脂质成分高效液相色谱-蒸发光散射检测(HPLC-ELSD)指纹图谱,将S1~S16的HPLC-ELSD指纹图谱数据导入中药色谱指纹图谱相似度评价系统(2012版),指认共有峰和评价相似度,采用HPLC-ELSD测定S1~S16中亚油酸、棕榈酸、油酸和麦角甾醇含量.结果表明:共指认4个共有峰,分别为亚油酸、棕榈酸、油酸和麦角甾醇;除S14外,其他15批灵芝孢子粉中脂质成分HPLC-ELSD指纹图谱相似度高,较为稳定;不同来源的灵芝孢子粉中亚油酸、棕榈酸和油酸的含量差异较大,麦角甾醇含量较为稳定.
译  名:
Fingerprint Analysis and Content Determination of Lipid Components in Ganoderma lucidum Spore Powder by HPLC-ELSD
关键词:
Ganoderma lucidum spore powder%HPLC-ELSD%lipid components%fingerprint
摘  要:
Sixteen batches of Ganoderma lucidum spore powder samples(S1-S16)from different sources were used to establish the chromatographic fingerprint of lipid components in G.lucidum spores by HPLC-ELSD.The HPLC-ELSD fingerprint data of S1-S16 were imported into the similarity evaluation system for chromatographic fingerprints of traditional Chinese medicine(2012 edition)to identify common peaks and evaluate similarity.Then the contents of common peaks in S1-S16 were determined by HPLC-ELSD.The results showed that the common peaks were identified as linoleic acid,palmitic acid,oleic acid and ergosterol.Except S14,the HPLC-ELSD fingerprints of the rest 15 batches of G.lucidum spore powder were highly similar.There were variations in the contents of linoleic acid,palmitic acid and oleic acid in S1-S1 6,whereas the ergosterol content was stable across the samples.

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