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Position: Home > Articles > Artificial Design Cecropins of AMP-D and Determination of Activity China Animal Husbandry & Veterinary Medicine 2012,39 (7) 165-168

人工设计天蚕素抗菌肽AMP-D及其活性测定

作  者:
韩晋辉;翟培;施用晖
单  位:
江南大学;广东食品药品职业学院
关键词:
天蚕素;AMP-D;抑菌活性;溶血性
摘  要:
本试验通过对抗菌肽结构改造优化,得到了一条新设计的抗菌肽AMP-D,并利用固相合成技术人工合成后对其活性和溶血性进行测定。试验结果表明,AMP-D对革兰氏阴性菌及真菌没有生物活性,对革兰氏阳性菌有明显的抑制作用,且其没有溶血活性;AMP-D对金黄色葡萄球菌的最小抑菌浓度为10.5μg/mL,较原设计肽活性有较大提高。
译  名:
Artificial Design Cecropins of AMP-D and Determination of Activity
作  者:
HAN Jin-hui1,ZHAI Pei1,SHI Yong-hui2(1.Guangdong Food and Drug Vocational College,Guangzhou 510520,China;2.Jiangnan University,Wuxi 214122,China)
关键词:
cecropin;AMP-D;antibacterial activity;hemolytic
摘  要:
By the experiment of antimicrobial peptides structure optimization and solid-phase synthesis technology of synthetic,new antibacterial peptide AMP-D could be obtained.According to determination of its activity and hemolytic,the experimental results showed that AMP-D could inhibit the growth of Gram-positive bacteria,and was no hemolytic activity.Minimal inhibitory concentration of AMP-D was 10.5 μg/mL against Staphylococcus aurous.The antimicrobial activities of AMP-D had more improve than the original peptide.

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