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Position: Home > Articles > QTL molecular marker location of gummy stem blight resistance in melon(Cucumis melo) Journal of Fruit Science 2013,30 (5) 30-34

甜瓜蔓枯病抗性QTL定位的研究

作  者:
刘龙洲;翟文强;陈亚丽;陈幼源;朱为民
单  位:
上海交通大学农业与生物学院;上海市农业科学院园艺研究所;上海市设施园艺技术重点实验室;新疆农业科学院哈密瓜研究中心;南京农业大学园艺学院
关键词:
甜瓜(Cucumis melo L.);蔓枯病;数量性状基因(QTL);F2:3家系
摘  要:
【目的】探讨甜瓜(Cucumis melo L.)蔓枯病抗性遗传规律并筛选出抗性连锁分子标记位点。【方法】高抗蔓枯病品系JGD-3做母本,感蔓枯病品系S717做父本,杂交构建包含122个单株的F2代群体,各单株分别自交得到相应的F3代家系;分别于2010年秋季和2011年春季叶片人工喷雾苗期接菌,完成蔓枯病抗性遗传规律鉴定;基于已构建对应分子标记连锁图谱,联合复合区间定位蔓枯病抗性数量基因位点(QTL)。【结果】两种环境下共检测到甜瓜蔓枯病抗性5个QTL(gsb1.1,gsb2.1,gsb3.1,gsb5.1和gsb6.1)作用位点,位于连锁群1,2,3,5和6上,单个QTL解释贡献率于2.5%~23.0%之间。两种环境中稳定重复检测的QTL是gsb1.1和gsb6.1;gsb2.1在2010年秋季被检测出,gsb3.1和gsb5.1在2011年春季检测到。两种环境下检测的QTL解释表型变异总和是42.0%(2010年秋季)和49.0%(2011年春季)。【结论】以甜瓜JGD-3为抗源鉴定蔓枯病抗性为数量遗传性状由多基因共同控制,有2个QTL位点在两种环境中被重复检测;与QTLs紧密连锁的标记(<5cM)为分子标记辅助选择(MAS)甜瓜抗蔓枯病品系和蔓枯病抗性基因分离、克隆提供了技术支撑。
译  名:
QTL molecular marker location of gummy stem blight resistance in melon(Cucumis melo)
作  者:
LIU Long-zhou;ZHAI Wen-qiang;CHEN Ya-li;CHEN You-yuan;ZHU Wei-min;Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Lab of Protected Horticultural Technology;Xinjiang Academy of Agricultural Sciences;College of Horticulture,Nanjing Agriculture University;
关键词:
Melon(Cucumis melo L.);;Gummy stem blight;;Quantitative trait loci(QTL);;F2:3 families
摘  要:
【Objective】The task of this research is to identify the genetic rule and find the QTLs of gummy stem blight(GSB) resistance in melon(Cucumis melo L.).【Method】 The melon lines,JGD-3(gummy stem blight(GSB) resistant) and S717(GSB susceptible),and their F2:3 populations were used to investigate GSB resistance though seedling inoculation in 2010/Autumn and 2011/Spring.QTL analysis was undertaken based on a constructed molecular linkage map of the corresponding F2 population by composite interval mapping.【Result】A total of five QTLs(gsb1.1,gsb 2.1,gsb 3.1,gsb 5.1 and gsb6.1) for GSB resistance on the linkage groups 1,2,3,5 and 6,respectively,were identified with explaining 2.5% ~23.0% of the phenotypic variation.Two consistent QTLs(gsb1.1 and gsb6.1) were detected under two conditions.The QTL gsb2.1 was only found in 2010/Autumn and QTLs gsb3.1 and gsb5.1 were identified in 2011/Spring.The total phenotypic variation showed by the QTLs was 42.0% and 49.0% in 2010/Autumn and 2011/Spring,respectively.【Conclusion】The studies showed that GSB resistance in melon exhibited an obvious characteristic of quantitative character inheritance,controlling by the poly-genes.Two consistent QTLs were repeatedly detected under two conditions.Anchor markers tightly linked to those loci(<5 cM) could lay a solid foundation for both molecular marker-assisted breeding and map-based gene cloning of the GSB-resistance gene in melon.

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