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当前位置: 首页 > 文章 > 水稻铵转运蛋白基因OsAMT1;4和OsAMT5的特征分析、功能和表达 中国农业科学 2007,40 (11) 2395-2402
Position: Home > Articles > Characterization, Function and Expression Analysis of Ammonium Transporter Gene OsAMT1;4 and OsAMT5 in Rice (Oryza sativa) Scientia Agricultura Sinica 2007,40 (11) 2395-2402

水稻铵转运蛋白基因OsAMT1;4和OsAMT5的特征分析、功能和表达

作  者：
邓若磊;谷俊涛;路文静;徐海荣;曹云飞;肖凯
单  位：
河北农业大学生命科学学院;河北农业大学农学院
关键词：
铵转运蛋白;OsAMT1;4;OsAMT5;异源互补;基因功能;基因表达
摘  要：
【目的】阐明水稻铵转运蛋白基因OsAMT1;4和OsAMT5的编码蛋白特征、功能和表达。【方法】采用生物信息学技术,揭示目的基因的编码蛋白特征;通过铵离子吸收缺陷的酵母突变体功能互补技术,鉴定上述基因编码蛋白的功能;采用RT-PCR技术,研究基因的表达特征。【结果】OsAMT1;4和OsAMT5的编码阅读框为1497bp和1377bp,分别编码498和458个氨基酸残基。OsAMT1;4和OsAMT5均具有11个跨膜域,分别归属与AMT1和AMT2亚家族。OsAMT1;4和OsAMT5均具有使NH4+吸收缺陷的酵母突变体重新恢复吸收NH4+的能力。OsAMT5在叶中特异表达,随NH4+浓度增加表达增强;在不同NH4+浓度和时间处理中,根、叶均未检测到OsAMT1;4的转录本。【结论】OsAMT1;4和OsAMT5在水稻中具有吸收或转运NH4+的功能。OsAMT5为叶特异表达,OsAMT1;4的表达受到NH4+因子外其他因素的调节。
译  名：
Characterization, Function and Expression Analysis of Ammonium Transporter Gene OsAMT1;4 and OsAMT5 in Rice (Oryza sativa)
作  者：
DENG Ruo-lei1, GU Jun-tao2, LU Wen-jing2, XU Hai-rong1, CAO Yun-fei1, XIAO Kai1 (1College of Agronomy, Agricaltural University of Hebei, Baoding 071001; 2College of Life Science; Agricultural University of Hebei, Baoding 071001)
关键词：
Ammonium transporter; OsAMT1;4; OsAMT5; Complementary analysis of gene function; Gene function; Gene expression
摘  要：
【Objective】To elucidate the characterization, function and expression pattern of two ammonium transporter genes OsAMT1;4 and OsAMT5 in rice (Oryza sativa). 【Method】The characterization of the encoded proteins by OsAMT1;4 and OsAMT5 was identified based on bioinformatics technology. The complementary analysis of function on NH4+ uptake for OsAMT1;4 and OsAMT5 was performed by genetic transformation of the yeast mutant lacking the ability of transporting NH4+. The gene expression patterns of OsAMT1;4 and OsAMT5 were analyzed by RT-PCR. 【Results】OsAMT1;4 and OsAMT5 had the open reading frame (ORF) 1 497 bp and 1 377 bp, encoded 498 amino acids and 458 amino acids, respectively. OsAMT1;4 and OsAMT5 belonged to AMT1 and AMT2 sub-family, all containing 11 conserved transmambrane domains, and could recover the mutant lacking the ability of transporting NH4+ to re-uptake NH4+ in the growth medium. The transcripts of OsAMT5 were only in leaves and the expression levels increased with the increase of NH4+ concentration in the medium. In all treatments, the transcripts of OsAMT1;4 in roots and leaves were all not detected.【Conclusion】OsAMT1;4 and OsAMT5 are involved in the uptake or transportation of NH4+ in the plants of rice. OsAMT5 is leaf specific in expression under NH4+ treatments. The expression of OsAMT1;4 is possibly regulated by other environmental conditions, rather than by NH4+.

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