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当前位置: 首页 > 文章 > 对二点委夜蛾高毒力苏云金芽胞杆菌的筛选及其基因型分析 应用昆虫学报 2014 (3) 36-41
Position: Home > Articles > Selection and genotype analysis of Bt isolates with high virulence against Athetis lepigone (Mschler) Chinese Journal of Applied Entomology 2014 (3) 36-41

对二点委夜蛾高毒力苏云金芽胞杆菌的筛选及其基因型分析

作  者：
杨云鹤;宋萍;王振营;董志平;王勤英
单  位：
河北农业大学;河北省农林科学院谷子研究所;中国农业科学院植物保护研究所
关键词：
苏云金芽胞杆菌;二点委夜蛾;基因型鉴定;杀虫活性;Vip3A蛋白;Cry1Ac蛋白
摘  要：
【目的】获得对二点委夜蛾Athetis lepigone(M(o|¨)schler)高毒力的苏云金芽胞杆菌(Bt)菌株,寻找对该虫具有特异杀虫活性的蛋白毒素,探索Bt菌株或其杀虫基因应用于二点委夜蛾防治的可行性。【方法】通过生物测定方法比较了36株苏云金芽胞杆菌和一株恶臭假单胞工程菌PHB-cry1Ab对二点委夜蛾幼虫的杀虫活性,同时利用PCR-RFLP方法对这些菌株的基因型进行了分析。【结果】不同Bt菌株对二点委夜蛾幼虫的杀虫活性差别很大,杀虫活性高的菌株都含有cry1Ac基因。饲毒72 h后含单基因的BtHD-73菌株(cry1Ac)对二点委夜蛾2龄幼虫的毒力(LC_(50)值为188.51μg/g)明显高于含多基因的Bt SC-40菌株(cry1Ac,cry2Ac,cry1I,vip3A)的毒力(LC_(50)值为418.13μg/g)。含有vip3A基因的Bt SC-40和BtHD-13营养期上清液对二点委夜蛾2龄幼虫表现出一定的杀虫活性(72 h死亡率分别达到42.5%和57.4%),而无vip3A基因的Bt HD-73营养期上清液未表现出明显的杀虫活性。【结论】由cry1Ac基因编码的Cry1Ac蛋白对二点委夜蛾幼虫具有特异杀虫活性,Vip3A蛋白对二点委夜蛾幼虫可能也有一定的杀虫活性。
译  名：
Selection and genotype analysis of Bt isolates with high virulence against Athetis lepigone (Mschler)
作  者：
YANG Yun-He;SONG Ping;WANG Zhen-Ying;DONG Zhi-Ping;WANG Qin-Ying;College of Plant Protection,Agricultural University of Hebei;Institute of Plant Protection,Chinese Academy of Agricultural Science;Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences;
关键词：
Bacillus thuringiensis;;Athetis lepigone;;genotype identification;;insecticidal activity;;Vip3A protein;;Cry1Ac protein
摘  要：
[Objectives]To obtain Bt strains and insecticidal proteins with high insecticidal activity against Athetis lepigone(Moschler).[Methods]The insecticidal activities of 36 Bt strains and an engineered Pseudomonas putida with a crylAb gene were evaluated on second instar larvae of A.lepigone by bioassay.PCR-RFLP analysis was performed to verify the genotypes of all Bt isolates.[Results]The bioassay results indicated obvious differences in the insecticidal activities of the different Bt isolates tested.The results also revealed that the Bt strains with high insecticidal activity all contained the crylAc gene.Furthermore,the results of a comparison of the toxicities of Bt SC-40(cry1Ac,cry2 Ac,cry1I,vip3A) and Bt HD-73(cry1Ac) showed that the LC_(50)of BT HD-73(188.51 μg/g) was lower than that of Bt SC-40(418.13 μg/g).The supernatants from the vegetative stage of Bt SC-40 and Bt HD-1 with the vip3 A gene showed certain insecticidal activities(42.5%and57.4%mortality,respectively) to second instar larvae of A.epigone.However,the supematants of Bt HD-73 with no vip3 A gene did not show obvious insecticidal activity.[Conclusion]These results imply that the CrylAc toxin encoded by the cry1 Ac gene play an important role in the resistance of plants to A.lepigone larvae.Vip3 A may also have some insecticidal properties with regard to this pest.

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