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Position: Home > Articles > Ultracytochemical Localization of ATP-ase Activity in The Cells of Jasminum Sambac L. Seedlings and Its Relation to Chilling Tolerance Acta Horticulturae Sinica 2001,28 (6) 544-550

茉莉幼苗ATP酶活性的超微细胞化学定位与耐冷性研究

作  者:
苏金为;王湘平
单  位:
福建农林大学测试中心;福建农林大学基础部
关键词:
茉莉;低温胁迫;超微结构;ATP酶
摘  要:
采用磷酸铅沉淀的细胞化学方法 ,电镜下观察两个抗冷力显著不同的茉莉品种幼苗ATP酶活性在细胞中超微结构定位及其经冷胁迫的变化。结果表明 ,抗寒力强的双瓣茉莉品种比抗寒力弱的单瓣品种在低温胁迫下超微结构和ATP酶活性有更强的适应能力。茉莉幼苗叶肉组织ATP酶主要定位于细胞质膜、液泡膜、叶绿体片层、线粒体脊和胞间连丝等部位 ,小叶脉维管束的ATP酶主要分布在筛管和伴胞
译  名:
Ultracytochemical Localization of ATP-ase Activity in The Cells of Jasminum Sambac L. Seedlings and Its Relation to Chilling Tolerance
作  者:
Su Jinwei 1 and Wang Xiangping 2 ( 1Department of Fundamental Subjects, Fujian Agriculture and Forestry University, Fuzhou 350002; 2 Centre of Test, Fujian Agriculture and Forestry University, Fuzhou 350002)
关键词:
Jasmine ( Jasminum Sambac L.); Low temperature stress; Ultrastructure; ATP ase
摘  要:
Ultracytochmical localization of ATP ase activity was carried out using a leading phosphate precipitation technique in the cells of two different chilling resistance Jasminum sambac L. Ait. seedlings. Under 0℃ chilling stress, the ultrastructure of cells and the ATP ase activity in the chilling resistant Jasmine (double petal cultivar) was obviously more stable than that in the chilling sensitive Jasmine(single petal cultivar). The ATP ase in Jasmine seedlings cells of mesophyll mainly located on plasmolemma, tonoplast, chloroplast lamellae, mitochondria cristae, and plasmodeslma ,whereas the ATP ase in cells of minor leaf vein mainly located on the sieve element and the companion cell.

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