中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 口蹄疫病毒Mya98谱系猪源及牛源2010年分离株的分子特征及蚀斑显型比较分析 中国兽医科学 2012 (4) 331-338
Position: Home > Articles > Molecular characteristics and plaque phenotype of Mya98/FMDV isolated from different hosts in 2010 Chinese Veterinary Science 2012 (4) 331-338

口蹄疫病毒Mya98谱系猪源及牛源2010年分离株的分子特征及蚀斑显型比较分析

作  者：
周强;包慧芳;白兴文;孙普;陈应理;谢宝霞;卢曾军;刘在新
单  位：
中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点实验室甘肃省生物检测工程技术研究中心国家口蹄疫参考实验室OIE口蹄疫参考实验室
关键词：
O型口蹄疫病毒;Mya98谱系;不同宿主;分子特征;蚀斑显型
摘  要：
为了明确口蹄疫病毒(FMDV)Mya98谱系猪源及牛源2010年分离株的分子特征及蚀斑显型差异,对采自不同宿主的4株FMDV进行了分子特征分析和蚀斑形态的比较。结果显示,来自不同宿主动物的分子特征差异主要表现为猪源毒株O/CHN/Mya98/2010S2与2个牛源毒株之间具有21个特异性的氨基酸置换位点,分散在ORF范围内。猪源毒株O/CHN/Mya98/2010S1具有与牛源毒株大量相似的氨基酸位点,并且与2株牛源毒株一样,都显示为小斑的蚀斑显型,而猪源毒株O/CHN/Mya98/2010S2在蚀斑试验中显示为大小斑混合存在。结果表明,猪源毒株O/CHN/Mya98/2010S2的变异位点对病毒蚀斑显型的改变具有重要意义。
译  名：
Molecular characteristics and plaque phenotype of Mya98/FMDV isolated from different hosts in 2010
作  者：
ZHOU Qiang,BAO Hui-fang,BAI Xing-wen,SUN Pu,CHEN Ying-li, XIE Bao-xia,LU Zeng-jun,LIU Zai-xin(State Key Laboratory of Veterinary Etiologic Biology/Key Laboratory of Animal Virology of the Ministry of Agriculture/National Foot-and-Mouth Disease Reference Laboratory of China/OIE Foot-and-Mouth Disease Reference Laboratory/Engineering Research Center of Biological Detection of Gansu Province/ Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China)
关键词：
FMDV serotype O;Mya98 strain;different host;molecular characterization;plaque phenotype
摘  要：
For understanding the molecular characteristics and plaque phenotype of Mya98/FMDV isolated from different hosts in 2010,molecular characteristic analysis and plaque phenotype comparison of 4 foot-and-mouth disease viruses(FMDV) isolated from various hosts were performed.And the result revealed that the isolates were of the swine strain O/CHN/Mya98/2010S2.This strain shared 21 specific amino acid substitution(AAS) sites with bovine strains,which were distributed within the range of the open reading frame(ORF).The swine strain O/CHN/Mya98/2010S1 also shared a number of similar amino acid sites with bovine strains,and both of the identified strains demonstrated small plaque phenotype.However,swine strain O/CHN/Mya98/2010S2 manifested as a mixture of small and large plaques in the plaque assay,suggesting that the variable site of this strain was of significance to the change in virus plaque phenotype.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46