作 者：
钟宇;陈壮壮;谢虹;燕飞;时红敏;张鹏远;王建光;陈穗云
单 位：
浙江省植物有害生物防控重点实验室——省部共建国家重点实验培育基地;云南大学生命科学学院植物科学研究所
关键词：
烟草悬浮细胞;青霉菌灭活菌丝体;防卫反应;诱导抗性;信号传导途径
摘 要：
青霉菌灭活菌丝体(Dry Mycelium of Penicillium chrysogenum,DMP)是工业生产青霉素的残余副产物,研究发现DMP能够提高多种作物的抗病性。本文研究了DMP对烟草BY-2悬浮细胞防卫反应的诱导作用,并初步探索其诱导机制,结果表明:DMP处理烟草BY-2悬浮细胞后,产生了活性氧迸发,在处理后30 min达到峰值;DMP诱导烟草BY-2悬浮细胞胞外基质碱性化,该变化能被蛋白激酶抑制剂K252a部分抑制;苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性被诱导而明显升高,分别在处理后4 h和8 h达到峰值;DMP诱导了病程相关蛋白基因PR-1a、PR-1b以及抗病信号传导途径关键基因NPR1的表达。说明DM P能够诱导烟草BY-2悬浮细胞产生抗病防卫反应,其抗病信号可能是通过水杨酸信号途径进行传导的,蛋白质磷酸化参与了该抗病信号的传导过程。
译 名：
Primary researches on dry mycelium of Penicillium chrysogenum-induced defense responses of tobacco BY-2 cell suspensions
作 者：
ZHONG Yu;CHEN Zhuang-zhuang;XIE Hong;YAN Fei;SHI Hong-min;ZHANG Peng-yuan;WANG Jian-guang;CHEN Sui-yun;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Institute of Virology and Biotechnology,Academy of Agricultural Sciences;Plant Science Institute of College of Life Science,Yunnan University;
关键词：
tobacco cell suspensions;;Dry Mycelium of Penicillium chrysogenum;;defense responses;;induced resistance;;signal pathway
摘 要：
Dry mycelium of Penicillium chrysogenum( DMP),a waste product of the pharmaceutical industry,was shown to enhance pathogen-resistance in many crops. The effects of DMP on defense responses in tobacco BY-2 cell suspensions and the mechanism of DMP-induced resistance were studied. The defense responses elicited by DMP in tobacco BY-2 cells included an oxidative burst, extracellular alkalinisation, changes of phenylalanine ammonia lyase( PAL) and peroxidase( POD) activities,and expression of three defense-related genes PR-1a,PR-1b and NPR1. The amount of H2O2 reached the highest level at 30 min after DMP-treatment. Alkalinisation response was induced by DMP in the cell suspensions,but reduced partially by protein kinase inhibitor K252 a. The enzyme activities of PAL and POD increased quickly after DMP-treatment,and reached the highest level at 4 h and 8 h,respectively. Our data suggest that DMP could induce a series of defense responses in tobacco BY-2 cell suspensions,in which SA-dependent signal pathway and protein phosphorylation events may be involved.
