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当前位置: 首页 > 文章 > 肉羊心型脂肪酸结合蛋白基因第2外显子多态性与肉质性状相关分析 东北农业大学学报 2013,44 (9) 30-34
Position: Home > Articles > Correlation analysis on polymorphisms and meat quality trait at the exon 2 of H-FABP gene in sheep Journal of Northeast Agricultural University 2013,44 (9) 30-34

肉羊心型脂肪酸结合蛋白基因第2外显子多态性与肉质性状相关分析

作  者：
曹阳;赵雪;于永生;张立春;郭振刚;金海国
单  位：
吉林省农业科学院畜牧分院;吉林农业大学动物科技学院;延边大学农学院
关键词：
肉羊;H-FABP基因;单核苷酸多态性;肉质性状
摘  要：
文章以杜泊×小尾寒羊为研究对象,采用PCR-SSCP方法对H-FABP基因exon2进行SNP检测,结果发现,g.939 A→G、g.980 G→A、g.1013 A→C、g.1018 T→C四个突变。与部分肉质性状、脂肪酸、氨基酸相关分析发现,基因型BB对剪切力有显著负效应(P<0.05),基因型BB对棕榈酸含量有显著正效应(P<0.05),基因型BB型对硬脂酸、亚油酸含量有极显著负效应(P<0.01),基因型BB对谷氨酸、丝氨酸、苏氨酸、丙氨酸含量以及氨基酸总和均有显著负效应(P<0.05),对脯氨酸含量有极显著负效应(P<0.01)。
译  名：
Correlation analysis on polymorphisms and meat quality trait at the exon 2 of H-FABP gene in sheep
作  者：
CAO Yang;ZHAO Xue;YU Yongsheng;ZHANG Lichun;GUO Zhengang;JIN Haiguo;Branch of Animal Husbandry,Jilin Academy of Agricultural Sciences;School of Agriculture,Yanbian University;School of Animal Science and Technology,Jilin Agricultural University;
关键词：
sheep;;H-FABPgene;;single nucleotide polymorphism(SNP);;meat quality trait
摘  要：
PCR-SSCP approach was applied to test the single nucleotide polymorphisms(SNPs) at the exon 2 of H-FABP(Heart fatty acid-binding protein) gene and Dorper×Small-Tailed Han sheep as the research object in this study. The results showed that four mutations(g.939 A→G,g.980 G→A,g.1013 A→C,g.1018 T→C) were detected. The correlation analysis on polymorphisms with part meat quality trait, fatty acids and amino acids showed that genotype BB had a negative effect on shear force(P<0.05), genotype BB had a significantly positive effect on palmitic acid content(P<0.05), genotype BB had a significantly negative effect on stearic acid and linoleic acid content(P<0.01), genotype BB had a certainly negative effect on glutamic acid, serine, threonine, alanine and the total amino acid(P<0.05) and had a significantly negative effect on proline content(P<0.01).

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