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当前位置: 首页 > 文章 > 核转录因子-κB在膀胱癌中作用机制的初步研究 石河子大学学报(自然科学版) 2005,23 (4)
Position: Home > Articles > Preliminary Study on Mechanism of NF-κB in Bladder Carcinoma Journal of Shihezi University(Natural Science) 2005,23 (4)

核转录因子-κB在膀胱癌中作用机制的初步研究

作  者：
张国玺;丁国富;王勤章;李锋;李洪安
单  位：
石河子大学医学院;石河子大学医学院一附院
关键词：
NF-κB;PKC;膀胱肿瘤;免疫组化;原位细胞凋亡检测
摘  要：
膀胱移行细胞癌石蜡包埋标本54例,正常对照标本13例。采用免疫组织化学SP法检测NF-κBP65和PKC蛋白在54例膀胱癌中的表达情况,运用TUNEL法原位检测凋亡细胞。分析NF-κB与PKC、细胞凋亡之间的关系,探讨NF-κB在膀胱癌中的可能作用机制。结果表明,NF-κBP65和PKC蛋白在54例膀胱癌标本中阳性表达率分别为:88.9%、81.5%,显著高于正常膀胱标本(P<0.01);54例膀胱癌中细胞凋亡的发生率为88.9%,凋亡指数均数为29.5741,均高于正常膀胱组织(P<0.01);NF-κBP65蛋白的表达与PKC蛋白的表达呈正相关(r=0.506,P<0.01),与细胞凋亡呈负相关(r=-0.390,P<0.01)。因此推论,NF-κB可能被PKC激活后,通过某种途径抑制细胞调亡,促进膀胱癌的发生和发展。
译  名：
Preliminary Study on Mechanism of NF-κB in Bladder Carcinoma
作  者：
ZHANG Guo-xi~1,DING Guo-fu~1,WANG Qin-zhang~1,LI Hong-an~2,LI Feng~2(1 1st Affiliated Hospital,College of Medicine,Shihezi University,Shihezi Xinjiang 832008,China;2 College of Medicine,Shihezi University,Shihezi Xinjiang 832002,China)
关键词：
NF-κB;PKC;bladder neoplasm;immunohistochemistry;apoptotic cell detection in situ
摘  要：
In order to study the mechanism of pathogenesis of bladder carcinoma,the expression of NF-κBP65 and PKC protein was detected in 54 cases of formlin-affixed,paraffin-embedded bladder transitional cell carcinoma by immunohistochemical SP method,and TDT-mediated Deoxyuridine Triphosphate-biotin nick end labeling(TUNEL) method was also used to detect apoptotic tumor cells.13 cases of normal bladder were assigned to controlled group.The correlation between NF-κB and PKC,NF-κB and cell apoptosis was analyzed,the possible mechanism of NF-κB in bladder carcinoma was explored.The results showed that the positive rates of NF-κBP65 and PKC protein in bladder transitional cell carcinoma were 88.9% and 81.5% respectively,and that in the controlled group were 7.7% and 7.7% respectively,the difference were significant(χ~2=31.146,P<0.01;χ~2=22.630,P<0.01).The rate of apoptotic bladder transitional cell carcinoma cells and the mean of AI were 88.9% and 29.5741 respectively,and in the controlled group were 15.4% and(0.6923) respectively,the difference were all significant(χ~2=26.140,P<0.01;t=5.496,P<0.01).NF-κBP65 protein was positively correlated with PKC protein in bladder transitional cell carcinoma(r=0.506,P<0.01),but was negtively associated with apoptotic tumor cells(r=-0.390,P<0.01).It is deduced that NF-κB is possibly activated by PKC,then inhibit the cell apoptosis,and promote the occurrence and progression of bladder carcinoma.

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