中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 7S β-伴大豆球蛋白通过NF-κB信号通路引起IPEC-J2细胞的炎性反应 畜牧兽医学报 2019 (4) 870-878
Position: Home > Articles > 7S β-conglycinin Triggered Inflammatory Response in IPEC-J2 Cells through NF-κB Signaling Pathway Chinese Journal of Animal and Veterinary Sciences 2019 (4) 870-878

7S β-伴大豆球蛋白通过NF-κB信号通路引起IPEC-J2细胞的炎性反应

作  者：
彭成璐;张瑜;丁雪东;李玉;冯士彬;王希春;李锦春;吴金节
单  位：
安徽农业大学动物科技学院
关键词：
β-伴大豆球蛋白;IPEC-J2细胞;NF-κB;PDTC;L-NAME
摘  要：
采用细胞体外培养技术,研究不同浓度7Sβ-伴大豆球蛋白对猪小肠上皮细胞(IPEC-J2细胞)的影响。试验分为A、B、C、D、E、F 6组,其中A为对照组,其余各组中分别添加1、5、10、5、5 mg·mL~(-1)的β-伴大豆球蛋白,并且在E和F组分别添加1μmol·L~(-1) NF-κB(PDTC)和iNOS(L-NAME)抑制剂。用CCK-8法检测各组细胞存活率,用ELISA法检测细胞NO、DAO、5-HT、IL-6和IL-10含量,用Western blot法检测细胞p-NF-κB p65、iNOS、COX-2的蛋白表达量,用qPCR法检测细胞NF-κB p65、IKKβ、iNOS、IKKα、COX-2 mRNA的相对转录量。结果显示:β-伴大豆球蛋白降低IPEC-J2细胞存活率,添加PDTC和L-NAME增高IPEC-J2细胞存活率,促进NO、DAO、5-HT和IL-6的分泌,并降低IL-10的分泌,同时增加p-NF-κB p65、iNOS、COX-2的蛋白表达量及NF-κB p65、IKKβ、iNOS、IKKα、COX-2 mRNA的相对转录量,添加PDTC和L-NAME后抑制了β-伴大豆球蛋白的作用。结果表明,β-伴大豆球蛋白引起IPEC-J2细胞损伤,随浓度增大损伤增加,PDTC和L-NAME可以降低β-伴大豆球蛋白对细胞的作用。
译  名：
7S β-conglycinin Triggered Inflammatory Response in IPEC-J2 Cells through NF-κB Signaling Pathway
作  者：
PENG Chenglu;ZHANG Yu;DING Xuedong;LI Yu;FENG Shibin;WANG Xichun;LI Jinchun;WU Jinjie;College of Animal Science and Technology, Anhui Agricultural University;
单  位：
PENG Chenglu%ZHANG Yu%DING Xuedong%LI Yu%FENG Shibin%WANG Xichun%LI Jinchun%WU Jinjie%College of Animal Science and Technology, Anhui Agricultural University
关键词：
β-conglycinin;;IPEC-J2;;NF-κB;;PDTC;;L-NAME
摘  要：
In this study, IPEC-J2 cells(porcine intestinal epithelial cells) were cultured in vitro to study the effect of different concentrations of 7 S β-conglycinin on IPEC-J2. The experiment was designed as six groups: A, B, C, D, E, and F, A was the control group, and the other groups were added 1, 5, 10, 5 and 5 mg·mL~(-1)β-conglycinin, respectively, and 1 μmol·L~(-1) NF-κB(PDTC) and iNOS(L-NAME) inhibitors were added to groups E and F, respectively. Cell viability was measured by CCK-8 method, the contents of NO, DAO, 5-HT, IL-6 and IL-10 were detected by ELISA, p-NF-κB, p65, iNOS and COX-2 protein expression levels were detected by Western blot, the relative transcription of NF-κB p65, IKKβ, iNOS, IKKα and COX-2 mRNA were determined by qPCR. The results showed that β-conglycinin reduced the viability of IPEC-J2 cells, promoted the secretion of NO, 5-HT and IL-6, decreased the secretion of IL-10, and increased the expression of p-NF-κB p65, iNOS and COX-2 proteins, transcription of NF-κB p65, IKKβ, iNOS, IKKα and COX-2 mRNA, PDTC and L-NAME can inhibit the effects of β-conglycinin. The results showed that β-conglycinin caused damage to IPEC-J2 and increased the damage with increasing concentration, PDTC and L-NAME can reduce the damage of β-conglycinin to cells.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46