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Position: Home > Articles > Induced Mutagenesis of Mortierella M11for Improving the Abilities to Produce Lipid and γ-Linolenic Acid FOOD SCIENCE 2010,31 (23) 322-325

产油脂和γ-亚麻酸被孢霉M11的诱变

作  者:
张超;徐洲;魏琴;李正国
单  位:
重庆大学生物工程学院;宜宾学院生命科学与食品工程学院
关键词:
被孢霉;紫外诱变;硫酸二乙酯诱变;复合诱变;油脂;γ-亚麻酸
摘  要:
为了提高被孢霉M11的油脂与γ-亚麻酸(GLA)含量,对M11菌株分别进行紫外(UV)、硫酸二乙酯(DES)、紫外与硫酸二乙酯复合(UV-DES)诱变的研究。结果表明:UV、DES、UV-DES复合诱变措施在一定程度上提高了突变菌株的油脂含量及GLA含量,但复合诱变比单一诱变更有效。突变株M117的油脂含量为41.3%,GLA含量9.28%,GLA产量1.52g/L,分别比出发菌株提高了11.26%、11.81%和24.59%。经9代连续传代培养,M117都能保持较稳定的产油和产γ-亚麻酸能力。
译  名:
Induced Mutagenesis of Mortierella M11for Improving the Abilities to Produce Lipid and γ-Linolenic Acid
作  者:
ZHANG Chao1,2,XU Zhou2,WEI Qin2,LI Zheng-guo1,(1. College of Bio-engineering, Chongqing University, Chongqing 400044, China; 2. Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan, College of Life Science and Food Engineering, Yibin University, Yibin 644000, China)
关键词:
Mortierella;UV mutagenesis;DES mutagenesis;combinatorial mutagenesis;lipid;γ-linolenic acid
摘  要:
In order to improve the productivity of lipid and γ-linolenic acid in Mortierella M11, the mutagenesis of Mortierella M11 was induced by UV irradiation, DES treatment and their combination, respectively. All the three mutant strains of Mortierella M11 obtained exhibited higher contents of lipid and γ-linolenic acid in comparison with it, and the combined induction was the most effective mutagenesis approach. The mutant strain M117 obtained through the combined induction of UV irradiation and DES treatment showed 41.3% lipid content, 9.28% GLA content and 1.52 g/L GLA yield, with incremental percentages of 11.26%, 11.81% and 24.59% as compared to the original strain, respectively. After nine generations of passage, the abilities of this stain to produce lipid and γ-linolenic acid were both kept stable.

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