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当前位置: 首页 > 文章 > 产低温蛋白酶海洋细菌的筛选及发酵条件 河北科技师范学院学报 2011,25 (3) 61-66
Position: Home > Articles > Screening and Fermentation Conditions of the Cold-active Protease Marine Strain Journal of Hebei Normal University of Science & Technology 2011,25 (3) 61-66

产低温蛋白酶海洋细菌的筛选及发酵条件

作  者：
郭艾英;凌云;张志雯
单  位：
中粮华夏长城葡萄酒有限公司;河北科技师范学院生命科技学院
关键词：
低温蛋白酶;筛选;发酵条件;酶活
摘  要：
采用平板初筛和摇瓶复筛等方法,从渤海秦皇岛海域的海水样品中筛选到产低温蛋白酶活力较高的菌株HYM-16,初步鉴定为黄色杆菌属,设计单因素和正交试验,确定该菌的最佳发酵条件。结果表明,在装液量50 mL液体培养基的250 mL三角瓶中,以体积分数0.01的接种量、摇床转速180 r/min条件下,25℃培养48 h酶活最高;最适碳源、氮源分别为蔗糖、豆饼粉,Mg2+显著提高产酶量;采用L9(34)正交试验得最适发酵培养基配方:蔗糖1 g/L,豆饼粉20 g/L,MgCl2 0.4 g/L,K2HPO4 0.5 g/L,KH2PO4 0.5 g/L。经验证,优化后的发酵培养基菌株酶活达605.8×103 U.L-1,较基础培养基提高了46.9%。
译  名：
Screening and Fermentation Conditions of the Cold-active Protease Marine Strain
作  者：
GUO Ai-ying1,LING Yun2,ZHANG Zhi-wen1(1 College of Life Science,Hebei Normal University of Science and Technology;2 COFCO Huaxia Great Wall Wine CO,LTD,Qinhuangdao Hebei,066600,China)
关键词：
cold-active protease;screening;fermentation condition;enzyme activity
摘  要：
Through preliminary petri-dish and following shaking flask fermentation screening,Strain HYM-16 producing higher protease was isolated from samples in Qinhuangdao of Bohai Sea located at 39°93′ N and 119°60′ E,and was primarily identified as Xanthobacter sp.Its fermentation condition was studied through the single-factor and orthogonal experiment.The results demonstrated that after 48 hours at 25 ℃ it had the maximum protease activity with 1% inoculation size and the stirring speed at 180 r/min,when 50 mL liquid culture in 250 mL flask.The optimal carbon and nitrogen source was saccharose and soybean flour respectively.Magnesium was beneficial to enzyme activity obviously.By using orthogonal experiment L9(34),the top-seeded formula for the media(g/L) was obtained: saccharose 1,soybean flour 20,Mg2+ 0.4,dipotassium hydrogen phosphate 0.5,and potassium dihydrogen phosphate 0.5.Finally,the optimized fermentation medium was validated,and the total activity of protesae was 605.8 U·mL-1,which was 46.9% higher than basic medium.

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