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Position: Home > Articles > 中麻黄叶绿体DNA序列不同引物扩增条件的筛选 Journal of Traditional Chinese Veterinary Medicine 2019 (3) 8-10

中麻黄叶绿体DNA序列不同引物扩增条件的筛选

作  者:
马晓辉;晋玲;朱田田
单  位:
甘肃中医药大学
关键词:
中麻黄;叶绿体;引物;条件筛选
摘  要:
目的:对16个不同居群的中麻黄从23对叶绿体编码区及非编码区DNA片段中筛选具有变异的叶绿体DNA片段.方法:通过试剂盒法提取DNA并梯度优化筛选扩增条件,PCR扩增产物经生物工程有限公司测序获得产物序列.结果:引物[trnS(GCU)-trnG(UCC)、atpB-rbcL、psbA-trnH、F71-R1516]能扩增出清晰、单一的条带.结论:16个不同居群中30个麻黄个体的叶绿体DNA扩增成功,初步筛选出具有变异的叶绿体DNA片段F71-R1516.
作  者:
MA Xiaohui;JIN Ling;ZHU Tiantian;Gansu University of Chinese Medicine;Key Laboratory of Chemistry and Quality for TCM and Tibetan Medicine in Universities of Gansu Province;Research Institute of Chinese(Tibetan)Medicinal Resources;
关键词:
Ephedra intermedia Schrenk et C. A. Mey;;chloroplast;;primer;;conditional screening
摘  要:
The Chloroplast DNA fragments with mutations from 23 pairs of chloroplast coding regions and non-coding regions were screened in 16 different populations of Ephedra intermedia. Methods:DNA was extracted and the amplification conditions were selected by gradient optimization. The PCR amplification products were sequenced by Bioengineering Co.,Ltd. to obtain the sequence.Results:The clear,single bands were obtained by primers(trnS(GCU)-trnG( UCC),atpB-rbcL,psbA-trnH,F71-R1516). Conclusion:Thirty individuals from 16 ephedra populations of chloroplast DNA were successfully amplified,and the chloroplast DNA fragments F71-R1516 with mutation were screened.

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