中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 不同保存方法对中麻黄基因组DNA提取效果的影响 山西农业科学 2013,41 (1) 23-25+59
Position: Home > Articles > Effect of Preservation Methods to Genomic DNA Extraction from Ephedra intermedia Journal of Shanxi Agricultural Sciences 2013,41 (1) 23-25+59

不同保存方法对中麻黄基因组DNA提取效果的影响

作  者：
朱田田;杜弢;晋玲;陈红刚;张延红;高素芳
单  位：
甘肃中医学院
关键词：
中麻黄;保存方法;DNA提取;效果
摘  要：
通过对不同方法采集和保存的中麻黄样品基因组DNA提取效果进行比较,确定中麻黄的最佳采集和保存方法。应用改良CTAB法提取中麻黄基因组DNA,并采用琼脂糖凝胶电泳检测其完整性,利用紫外分光光度计法测定其浓度和纯度。结果表明,新鲜样品采集后立即冷冻保存或置于液氮和硅胶后冷冻保存,均能提取出高质量的基因组DNA;鲜样阴干几天后冷冻保存会导致基因组DNA降解;阴干后在室温下保存则降解更严重。采集的中麻黄鲜样采取不同方法带回实验室均不影响基因组DNA的提取效果,但应在低温环境中保存才能有效防止基因组DNA的降解。
译  名：
Effect of Preservation Methods to Genomic DNA Extraction from Ephedra intermedia
作  者：
ZHU Tian-tian1,2,DU Tao1,3,JIN Ling1,2,CHEN Hong-gang1,3,ZHANG Yan-hong1,3,GAO Su-fang1,3(1.Gansu College of Traditional Chinese Medicine,Lanzhou 730000,China;2.Key Laboratory of Chemistry and Quality for Traditional Chinese Medicines,College of Gansu Province,Lanzhou 730000,China;3.Institute of Medicinal Plant Genetic Breeding,Gansu College of Traditional Chinese Medicine,Lanzhou 730000,China)
关键词：
Ephedra intermedia;preservation methods;DNA extraction;effect
摘  要：
The effect of genomic DNA extraction from Ephedra intermedia with different collection and preservation methods was compared and the best method was confirmed.The genomic DNA was extracted by improved CTAB method,the integrity,yield and purity of total genomic DNA were examined by UV-spectrophotomoter and agarose gel electrophoresis.High quality genomic DNA could be extracted from freshly frozen samples,whether the samples were frozen immediately or preserved in liquid nitrogen and silica gel after collection.The genomic DNA of fresh samples would degrade,which were frozen after drying a few days,and it was more serious that fresh samples were preserved in room temperature.The quality of genomic DNA would not be affected,which was brought back to laboratory by using different preservation methods,and cryopreservation could prevent genomic DNA degradation effectively.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46