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当前位置: 首页 > 文章 > 用淘汰奶牛卵巢生产体外受精奶牛胚胎的研究 中国牛业科学 2007 (2) 13-15
Position: Home > Articles > In Vitro Production of Cattle Embryos Derived from Abattoir Ovaries China Cattle Science 2007 (2) 13-15

用淘汰奶牛卵巢生产体外受精奶牛胚胎的研究

作  者：
田允波;曾书琴;李万利
单  位：
广东仲恺农业技术学院动物科学系
关键词：
淘汰奶牛;体外受精;收集方法;卵丘细胞;颗粒细胞
摘  要：
本文研究了淘汰奶牛卵巢采集方法、卵丘细胞和颗粒细胞对卵母细胞体外受精后发育的影响。结果表明奶牛屠宰后取其卵巢,用剖解法可比抽吸法得到更多的可用卵母细胞(8．5: 6．2枚／卵巢,P＜0．05)。经成熟培养和体外受精后,无卵丘细胞包围的裸露卵母细胞卵裂率为40．2%,但仅28．2%停留在8～16细胞期,最高发育阶段为16细胞期;而有3层以上卵丘细胞紧密包围的卵丘—卵母细胞复合体,其卵裂率(60．2%:53．2%,P＜0．05)和囊胚发育率(31．5%:19．6%,P＜0．05)均高于卵丘细胞包被不全的卵母细胞。在培养液中添加颗粒细胞,提高囊胚细胞数(96．2±5．2:70．4±4．6,P＜0．05),但没有促进牛受精卵的卵裂率。
译  名：
In Vitro Production of Cattle Embryos Derived from Abattoir Ovaries
作  者：
TIAN Yun-bo ZENG Shu-qin LI Wan-li (Department of Animal Science,Zhongkai Uuiversity of Agriculture and Technology,Guangzhou,Guangdong 510225,China)
关键词：
Culled cow;;IVF;;Collecting method;;Cumulus cells;;Granulose cells
摘  要：
This experiment was carried out to study the collect methods of abattoir ovaries,and the ef- fects of cumulus cells and granulose cells on developmental competence of bovine oocytes derived from a- battoir ovaries.The result showed that more available oocytes could be obtained in dissected than aspirated method(8.5/ovary vs 6.2/ovary,P＜0.05)after collecting ovaries from slaughtered cows.Following in vitro maturation,fertilization and culture(IVMFC),the cleavage rate of oocytes without cumulus cells (naked oocytes)from the follicles was 40.20% with only 28.2% at 8-16 cell stage,the best development was at 16-cell stage;the cleavage rates and blastocyst rates of cumulus and oocytes complex surrounded by more then 3-layer cumulus cells were significantly higher than that of oocytes partly surrounded by cu- mulus cells(60.2% vs 53.2%;31.5% vs 19.6%,P＜0.05).Addition of granulose cells in the culturing solution could increase the number of blastocytes(96.2±5.2:70.4±4.6,P＜0.05),but had not acceler- ated the cleavage rate of bovine embryos.

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