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Position: Home > Articles > Cloning and Sequence Analysis of the VP2 Gene of Porcine Parvovirus HN-2011 Strain Journal of Henan Agricultural Sciences 2013,42 (6) 130-133

猪细小病毒HN-2011株VP2基因的克隆及序列分析

作  者:
苌生科;马莉芳;伍锐;白朝勇;闫果;田克恭;张许科;王延辉
单  位:
国家兽用药品工程技术研究中心;河南农业大学牧医工程学院
关键词:
猪细小病毒;VP2基因;克隆;序列分析
摘  要:
根据GenBank中登录的猪细小病毒(PPV)VP2基因序列设计引物,扩增HN-2011河南分离株的VP2基因片段,并将其克隆到pMD 18T载体中。序列分析结果表明,HN-2011分离株VP2基因长度为1 740bp,编码580个氨基酸。利用MAGE 5.0软件绘制PPV系统发育进化树,结果显示,PPV毒株可以划分为4组,我国的PPV毒株主要集中在A和B两组,仅JY毒株位于C组;PPV VP2蛋白3维结构显示,氨基酸变异的多态性位点主要集中在病毒衣壳蛋白的表面。以上遗传进化关系表明,PPV HN-2011株是我国PPV的流行毒株。
译  名:
Cloning and Sequence Analysis of the VP2 Gene of Porcine Parvovirus HN-2011 Strain
作  者:
CHANG Sheng-ke1,MA Li-fang1,WU Rui2,BAI Chao-yong2,YAN Guo1, TIAN Ke-gong2,ZHANG Xu-ke1,2,WANG Yan-hui2(1.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China; 2.National Research Center for Veterinary Medicine,Luoyang 471003,China)
关键词:
PPV;VP2 gene;cloning;sequence analysis
摘  要:
According to the VP2 gene sequence of porcine parvovirus(PPV) published in GenBank database,a pair of specific primers was designed and synthesized.The full length VP2 gene of ppv HN-2011 isolate was amplified by PCR and then cloned into pMDl8-T vector.As expectedly,the complete nucleotide sequence of VP2 gene was 1 740 nt and coded 580 amino acids.The phylogenetic tree showed that PPV isolates were divided into four groups,and the Chinese isolates were clustered in groups A and B,except for the JY strain in group C.Mapping polymorphic sites of protein data onto the three-dimensional(3D) structure of PPV VP2 revealed that almost all substitutions were located on the external surface of the viral capsid.According to the phylogenetic relationship of the VP2 gene,the isolate HN-2011 was a prevalent PPV strain in China.

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