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当前位置: 首页 > 文章 > MSPD-HPLC法快速测定水果·蔬菜中常用的7种农药残留 安徽农业科学 2015 (21) 274-276+279
Position: Home > Articles > Rapid Determination of Seven Commonly-used Pesticides in Fruits and Vegetables by MSPD-HPLC Journal of Anhui Agricultural Sciences 2015 (21) 274-276+279

MSPD-HPLC法快速测定水果·蔬菜中常用的7种农药残留

作  者：
刘柱;陈万勤;茅佩卿;丁宇琦;朱炳祺;周赛静;罗金文
单  位：
浙江省食品药品检验研究院
关键词：
农药残留;高效液相色谱;基质分散固相萃取
摘  要：
[目的]建立同时检测水果、蔬菜中7种常用农药残留的基质分散固相萃取(MSPD)-高效液相色谱法(HPLC)分析方法。[方法]供试样品通过乙腈高速均质提取,经PSA和C18(2∶1)基质分散固相萃取快速净化,以C18柱进行HLPC紫外多波长检测。通过流动相、吸附剂种类和用量的选择,加标回收试验,建立和优化了MSPD-HPLC同时测定7种常用农药残留的方法。[结果]试验表明,7种常用农药在各浓度范围内呈良好的线性关系,且相关系数R均大于0.999;选取苹果、草莓、番茄、黄瓜4种具有代表性水果、蔬菜进行加标回收试验,回收率均大于80%,相对标准偏差(RSD)为5%以内,检出限(LOD,S/N=3)为0.01~0.06 mg/kg。[结论]研究表明,该MSPD-HPLC方法操作简单、快速、准确,可满足多种水果、蔬菜中7种常用农药残留同时检测的实际需要。
译  名：
Rapid Determination of Seven Commonly-used Pesticides in Fruits and Vegetables by MSPD-HPLC
作  者：
LIU Zhu;CHEN Wan-qin;MAO Pei-qing;LUO Jin-wen;Zhejiang Institute for Food and Drug Control;
关键词：
Multi-residue of pesticides;;High performance liquid chromatography(HPLC);;Matrix solid phase dispersion(MSPD)
摘  要：
[Objective] A method was developed for the simultaneous determination of seven commonly-used pesticides in fruits and vegetables by using matrix solid phase dispersion( MSPD)-high performance liquid chromatography( HPLC). [Method]The samples were extracted with acetonitrile,cleaned up by primary secondary amine( PSA) and C18( 2∶1) adsorbents with MSPD method,separated by C18 column via HPLC,analyzed by different UV wavelengths. Through the selection of mobile phase test,adsorbent selection test,adsorbent dosage test and recovery test,the method of MSPD-HPLC for the determination of seven commonly-used pesticides analysis was established and optimized.[Result]The result show that the calibration curves of seven commonly-used pesticides were linear in each concentration ranges with correlation more than 0. 999. The absolute recoveries of seven pesticides analytes from four common representative samples were more than 80%,with RSDs( n = 6) less than 5%. The limit of detection( LODs,S / N = 3) were 0. 01- 0. 06 mg / kg. [Conclusion] The MSPD-HPLC method is simple,rapid,accurate,which can meet the demands of practical used for the simultaneous determination of multi-residue pesticides in fruits and vegetables.

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