摘 要：
以桔梗茎为试材,采用微波协同双水相的方法,研究了各因素对桔梗茎总皂苷萃取率的影响,并考察了桔梗茎总皂苷提取液的体外抗氧化活性。结果表明:微波协同双水相萃取桔梗茎总皂苷的最佳工艺条件为料液比1∶25g/mL,微波功率600W,醇水比(mL∶mL)0.77,萃取时间7min,硫酸铵质量浓度0.21g/mL,在此工艺条件下,其平均萃取率为92.31%;桔梗茎总皂苷提取液具有一定的抗氧化能力,而且其抗氧化活性随桔梗茎总皂苷提取液质量浓度的增加而逐渐提高。
译 名：
Study on Extraction Process of Total Saponins in Platycodon grandiflorum Stems With Aqueous Two-phase Cooperated With Microwave Method and Its Antioxidant Ability
作 者：
ZHONG Fangli;WANG Wenjiao;WANG Xiaolin;ZHAN Honghe;School of Chemistry and Pharmaceutical Engineering,Jilin Institute of Chemical Technology;School of Chemistry,Jilin University;
关键词：
Platycodon grandiflorumstems;;aqueous two-phase;;total saponins;;antioxidant
摘 要：
Using Platycodon grandiflorum stems as experimental material,aqueous two-phase assisted with microwave method was applied to explore the influence of many factors on the total saponins extraction efficiency and its antioxidant ability in vitro.The results showed that the optimum ratio between material and solvent,the microwave power,the ratio between ethanol and water,extraction time and the mass concentration of ammonium sulfate were1∶25g/mL,600 W,0.77,7 min and 0.21g/mL,respectively.Under optimum process condition,the average total saponins extraction efficiency reached 92.31%.The activity test showed that total saponins extraction solution from Platycodongrandiflorum stems had certain antioxidant ability in vitro,which might gradually enhance the clearance ratio with the increase of mass concentration of total saponins in extraction solution.
