当前位置: 首页 > 文章 > 烟草野火病菌的快速检测 西北农林科技大学学报(自然科学版) 2002,30 (6) 138-143
Position: Home > Articles > Studies on rapid detection of tobacco wildfire(Pseudomonas syringae pv.tabaci) in Yunnan Journal of Northwest A & F University(Natural Science Edition) 2002,30 (6) 138-143

烟草野火病菌的快速检测

作  者:
刘雅婷;张世珖;李永忠;王绍坤
单  位:
云南农业大学农学与生物技术学院
关键词:
烟草野火病;烟草野火病菌;快速检测;间接-ELISA;SPA-ELISA;凝集反应
摘  要:
 对采自云南各大烟区的烟草野火病菌株进行了分离、鉴定,用通过筛选致病力强的烟草野火病菌株为抗原,制备了烟草野火病菌特异性的抗血清,建立了一套快速检测烟草野火病菌的方法,制备了SPA-ELISA反应试剂盒,应用玻片凝集或协同凝集反应与SPA-ELISA反应试剂盒相结合的方法,使检测真正做到了简便、快速、灵敏、准确。
译  名:
Studies on rapid detection of tobacco wildfire(Pseudomonas syringae pv.tabaci) in Yunnan
作  者:
LIU Yating1,ZHANG Shiguang2*,LI Yongzhong3,WANG Shaokun4(1 Faculty of Agricultural Science and Technology;2 Phytopathology Laboratory of Yunnan Province;3 Faculty of Tabacco,Yunnan,Kunming 650201,China;4 Qujing Tobacco Company,Yunnan Provience,Qujing,Yunnan 655001,China)
关键词:
tobacco wildfire;Pseudomonas syringae pv.tabaci;rapid detection;indirect-ELISA;SPA-ELISA;agglutination
摘  要:
Strains of tobacco wildfire from Yunnan were isolated and identified.Pseudomonas syringae pv.tabaci specific antiserum was obtained through screening the strongvirulent strains as antigen.Straphylococal protein A ELISA (SPAELISA) kits were developed successfully and this was the first time to establish the rapid detecting method of the pathogen.Detection techniques become easier,rapider,more sensitive and accurate,by combining SPAELISA with agglutination test (slide agglutination and coagglutination).

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