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Position: Home > Articles > Isolation and Screening of Phosphate-solubilizing in the Rhizosphere of Soybean Hubei Agricultural Sciences 2014,53 (13) 3048-3050

大豆根际解磷细菌的分离及筛选

作  者:
李丽丽;郎敬;杨洪一;邰飞飞;来永才
单  位:
沈阳农业大学园艺学院;黑龙江省农业科学院耕作栽培研究所;东北林业大学生命科学学院;黑龙江省农业科学院耕作栽培研究所东北林业大学博士后科研流动站/黑龙江省农业科学院博士后科研工作站黑龙江省林业科学研究所东北林业大学生命科学学院
关键词:
解磷细菌;溶磷量测定;大豆
摘  要:
解磷菌对于提高土壤中可溶性磷的含量、促进植物对磷的吸收具有重要作用。利用PVK培养基对黑龙江省海伦、北安地区大豆根际土壤中的解磷细菌进行初筛,共分离得到8株具有解无机磷能力的菌株。利用NBRIP培养基进行摇瓶复筛,培养3 d后,根据摇瓶中上清液的可溶性磷量的高低,筛选出5株解磷能力较强的菌株。其中海伦Ⅳ号的解无机磷能力最强,其培养液上清中的溶磷量可提高约10倍。研究中的技术体系可有效获得溶磷的细菌菌株,为进一步开发细菌磷肥打下了基础。
译  名:
Isolation and Screening of Phosphate-solubilizing in the Rhizosphere of Soybean
作  者:
LI Li-li;LANG Jing;YANG Hong-yi;TAI Fei-fei;LAI Yong-cai;Crop Tillage and Cultivation Institute, Heilongjiang Academy of Agricultural Sciences;Northeast Forestry University Postdoctoral Programme, Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme;Institute of Forestry Science of Heilongjiang Province;College of Life Science, Northeast Forestry University;
关键词:
phosphate-solubilizing bacteria;;determination of phosphorus content;;soybean
摘  要:
The phosphate-solubilizing bacteria in the soil play an important role for improving the phosphorus level in the soil and absorbing the phosphorus nutrition for plants. The phosphate-solubilizing bacteria(PSB) in the rhizosphere of soybean was isolated using modified PVK medium. Eight strains of PSB were isolated. Subsequently, 5 strains of high-effective PSB were isolated according to phosphorus level in supernatant of culture solution after 3-day incubation in a liquid medium NBRIP. Hailun Ⅳ was found to possess the highest phosphate-solubilizing ability among 5 strains under the screening conditions. The soluble P in the supernatant of the culture of Hailun Ⅳ increased about 10-fold. The screening system can efficiently obtain PSB and is helpful to develop PSB phosphorus fertilizer.

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