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Position: Home > Articles > Screening and Promotion Effect of Phosphate-solubilizing Bacteria from Grapevine Rhizosphere in Weibei Semi-arid Plateau Acta Agriculturae Boreali-occidentalis Sinica 2009,18 (4) 185-190

渭北旱塬葡萄根际解磷细菌的筛选及其对葡萄的促生效应

作  者:
程宝森;房玉林;刘延琳;屈雁朋;宋士任
单  位:
西北农林科技大学葡萄酒学院;陕西省葡萄与葡萄酒工程技术研究中心
关键词:
解磷细菌;葡萄;促生效应
摘  要:
对渭北旱塬50年生葡萄园解磷细菌进行了筛选,并对两个菌株的促生效应进行了研究。通过NBRIP平板,从葡萄根际土壤中分离得到29株解磷细菌。通过NBRIP液体摇瓶试验,培养7 d后得到两株解磷效果突出的菌株:JYR-12菌株培养液中有效磷含量高达673.99 mg/L,约有13.17%的磷酸钙被溶解出来;JYR-7菌株培养液中有效磷浓度为671.64 mg/L。在盆栽条件下,研究解磷细菌JYR-12和JYR-7对酿酒葡萄赤霞珠扦插苗生长及光合能力的影响。盆栽试验表明,接种解磷细菌处理的赤霞珠扦插苗株高、茎粗、地下干质量和地上干质量显著高于对照。葡萄扦插苗的光合指标及接种解磷细菌的均显著高于对照。可见,与对照相比,接种解磷细菌可以显著促进赤霞珠扦插苗生长,其中以菌株JYR-12效果最为明显。
译  名:
Screening and Promotion Effect of Phosphate-solubilizing Bacteria from Grapevine Rhizosphere in Weibei Semi-arid Plateau
作  者:
CHENG Baosen1,FANG Yulin1,2,LIU Yanlin1,2,QU Yanpeng1 and SONG Shiren1(1.College of Enology,Northwest A&F University,Yangling Shaanxi 712100,China;2.Shaanxi Engineering Research Center for Viti-viniculture,Yangling Shaanxi 712100,China)
关键词:
Phosphate-solubilizing bacteria;Grapevine;Growth promotion
摘  要:
Phosphate-solubilizing bacteria were screened and promotion effects on grapevine were studied.By using NBRIP plate method,29 strains of phosphate-solubilizing bacteria(PSB) were isolated from the grapevine rhizosphere.After seven days incubation in a liquid medium NBRIP,the soluble P in the cultures of strains JYR-12 and JYR-7 reached 673.99 mg/L and 671.64 mg/L respectively.About 13.17% of tricalcium phosphate was dissolved.In pot experiment,the growth of Cabernet sauvignon after inoculation with phosphate-solubilizing bacteria was studied.The results showed that the plant height,stem thickness,shoot dry weight and root dry weight were significantly higher in PSB treatments than in CK.After the inoculation with phosphate-solubilizing bacteria,photosynthesis indexes of grape were obviously higher than those of contrast plants.In conclusion,the inoculation of phosphate-solubilizing bacteria could significantly promote the growth of Cabernet sauvignon.Among all these inoculation methods,the best is JYR-12.

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