中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 一个八倍体小偃麦染色体组成的分子细胞学分析及品质特性鉴定 麦类作物学报 2006,26 (2) 13-17
Position: Home > Articles > Molecular Cytogenetic Analysis and Quality Characteristics Identification of an Introduced Octoploid Trititrigia Journal of Triticeae Crops 2006,26 (2) 13-17

一个八倍体小偃麦染色体组成的分子细胞学分析及品质特性鉴定

作  者：
李伟光;王志国;纪军;王静;李俊明
单  位：
中国科学院遗传与发育生物学研究所农业资源中心
关键词：
八倍体小偃麦;基因组原位杂交;高分子量麦谷蛋白亚基
摘  要：
八倍体小偃麦是普通小麦遗传改良的重要种质材料,为进一步发掘和利用其优良基因,对从匈牙利科学院农业研究所引进的八倍体小偃麦BE-1的染色体组成和高分子量麦谷蛋白亚基(HMW-G S)进行了分析鉴定。细胞学观察结果表明,其体细胞染色体数目为2n=56,花粉母细胞减数分裂中期Ⅰ(PM C M I)染色体构型2n=28Ⅱ的细胞占65.8%;分别用长穗偃麦草(T h inopy rum elong a tum,D.R D ew ey,2n=14,EE)、百萨薄冰草(T h inopy rum bessarabicum,A.Love,2n=14,JJ)、假鹅观草(P seud oroegneria strig osa,A.Love,2n=14,S tS t)的总基因组DNA作探针进行原位杂交,结果发现仅在假鹅观草总基因组DNA作探针时,能够检测到BE-1 14条染色体明显的杂交信号,进而认为它们来源于S t基因组相近的鹅观草属的物种;对八倍体小偃麦BE-1麦谷蛋白进行SDS-PAGE分析,结果表明其HMW-G S组成为1,6+8,5+10。红外谷物品质分析仪测试结果表明,它的蛋白质、湿面筋含量及沉淀值均超过当前优质小麦品种,可做为普通小麦品质改良的重要基因源。
译  名：
Molecular Cytogenetic Analysis and Quality Characteristics Identification of an Introduced Octoploid Trititrigia
作  者：
LI Wei-guang~(1,2),WANG Zhi-guo~1,JI Jun~1,WANG Jing~1,LI Jun-ming~1(1.Research Center of Agricultural Resource,Institute of Genetics and Development Biology,Chinese Academy of Sciences,Shijiazhuang 050021,China;2.Graduate School,Chinese Academy of Sciences,Beijing 100039,China)
关键词：
Octoploid Trititrigia;Genomic in situ hybridization;HMW-GS
摘  要：
Octoploid Trititrigia is an important germplasm for wheat genetic improvement.In order to exploit its benefit genes,one of the Octoploid Trititrigia line BE-1 was introduced from the Agricultural Institute of Hungary Sciences.Its chromosome constitution and HMW-GS was studied in the paper.The cytogenetic results indicated that the chromosome number of BE-1 was 2n=56 in root tip cells and the chromosome configuration of 65.8% cells at the PMC MI were 2n=28Ⅱ;furthermore,genomic in situ hybridization(GISH) using genomic DNA from Thinopyrum elongatum,D.R Dewey(E.E,2n=14)、Thinopyrum bessarabicum,A.Love(JJ,2n=14)、Pseudoroegneria strigosa.A.Love(StSt,2n=14),was used to examine the genomic constitution of BE-1.Evidence of GISH showed the only St genome could distinguished 14 home-rogenous chromosomes with distinct hybrid signal,while the E and J genome had only indistinct and partial hybrid patterns.It indicated that the heterogenous chromosomes of BE-1 was more closer to that of Pseudoroegneria strigosa,and the genome constitution of BE-1 was primarily thought to be AABBDDStSt.The analysis of HMW-GS of BE-1 by SDS-PAGE showed it consisted of 1,6+8,5+10 subunits,seed quality analysis indicated its protein content,wet gluten index and sedimentation value exceeded other Octoploid Trititrigia Zhong 1~Zhong 7 and Xiaoyan 6 and Gao 8901.In conclusion Octoploid Trititrigia BE-1 should be an important genome resources in wheat improvement.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46