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当前位置: 首页 > 文章 > 金叶络石离体培养植株再生及其栽培试验 北方园艺 2010 (21) 180-182
Position: Home > Articles > Rssearch on in vitro Culture for Plant Regeneration and Cultivation of Ornamental of Trachelospermum asiaticum cv.Ougonnishiki Northern Horticulture 2010 (21) 180-182

金叶络石离体培养植株再生及其栽培试验

作  者：
邹清成;周江华;朱开元;刘慧春
单  位：
浙江省农业科学院花卉研究开发中心
关键词：
金叶络石;离体培养;植株再生;栽培;观赏植物
摘  要：
以金叶络石茎段为外植体,分别接种于添加不同浓度6-BA、NAA的MS培养基中,设置不同的温度环境、光照强度、肥料、基质栽培试验,观察生发育情况。结果表明:诱导愈伤组织最佳的培养基为MS+BA 3.0 mg/L+NAA 0.2 mg/L+蔗糖3%+琼脂0.5%;丛生芽分化增殖的最佳培养基为MS+BA 1.5 mg/L+NAA 0.2 mg/L+3%蔗糖+0.5%琼脂;生根的最佳培养基为1/2MS+NAA 0.2 mg/L+蔗糖3%+琼脂0.5%。栽培观赏研究表明:盆栽最佳温度为20~30℃;最佳光照强度为80%;最佳基质为纯泥炭或者泥炭+蛭石(3∶1);最佳肥料配比为N∶P∶K=1∶1∶1。
译  名：
Rssearch on in vitro Culture for Plant Regeneration and Cultivation of Ornamental of Trachelospermum asiaticum cv.Ougonnishiki
作  者：
ZOU Qing-cheng,ZHOU Jiang-hua,ZHU Kai-yuan,LIU Hui-chun (Research and Development Center of Flower,Zhejiang Academy of Agricultural Sciences.Xiaoshan,Zhejiang 311202)
关键词：
Trachelospermum asiaticum cv.Ougonnishiki;in vitro culture;regenaration;cultivation;ornamental
摘  要：
In vitro culture and plant regenaration of Trachelospermum asiaticum cv.Ougonnishiki by tissue culture using its blade were studied.Inoculated the blade into MS medium and with different concerdration of BA and NAA.Observed growth condition under different temperature,illumination,medium and fertilizer.The results showed that the optimum medium for callus induction was MS supplemented with BA 3 mg/L,NAA 0.2 mg/L,3% sucrose and 0.5% agar.The optimum medium for the formation and growth of cluster buds was MS supplemented with BA 1.5 mg/L,NAA 0.2 mg/L,3% sucrose and 0.5% agar.The optimum medium for rooting was 1/2 MS with NAA 0.2 mg/L,3% sucrose and 0.5% agar.The cultivation for ornamental of test-tube was also studied.The optimum temperature was 20～30℃.The optimum light intensity was 80%.The optimum medium was peat or 2/3 peat and 1/3 vermiculite.The best proportion of N,P and K was 1∶1∶1.The test-tube plantlets can be cultivated as a pot flower and ornamental lawn with high ornamental value.

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