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Position: Home > Articles > Rapid Propagation of Arnebia euchroma(Royle.) Xinjiang Agricultural Sciences 2010,47 (4) 726-730

新疆紫草快繁技术研究

作  者:
郝爱花;芦韦华;陈永芳;程琼浩;孙新华;王芳
单  位:
新疆农业大学;山东省邹城第四中学
关键词:
新疆紫草;离体快繁;玻璃化
摘  要:
【目的】建立新疆紫草组培快繁技术体系。【方法】以新疆紫草无菌苗的茎尖为外植体,筛选适合茎尖萌发、增殖、生根的激素组合。【结果】茎尖最佳萌发培养基为MS+TDZ 1.0 mg/L,其萌发率为100%。芽增殖培养基为MS+TDZ 1.0 mg/L,芽增殖倍数为13.50倍,继代壮苗培养基为MS+NAA 0.3 mg/L+6-BA 0.5mg/L,生根培养基为MS+NAA 0.1 mg/L,生根率为84%。降低6-BA浓度到0.05 mg/L可大幅降低玻璃化发生率。经过此培养基继代后,再在原生根配方中生根,生根率提高到95.32%。【结论】茎尖是新疆紫草快繁的最佳外植体。
译  名:
Rapid Propagation of Arnebia euchroma(Royle.)
作  者:
HAO Ai-hua1,LU Wei-hua1,CHEN Yong-fang2,CHENG Qiong-hao1,SUN Xin-hua1,WANG Fang1(1.Xinjiang Agricultural University,Urumqi 830052,China;2.Middle School No.4 in Zoucheng,Zouheng Shandong,255000 China)
关键词:
Arnebia euchroma(Royle.)Johnst;rapid propagation in vitro;vitrification
摘  要:
【Objective】The aim of the study was to establish rapid propagation system of Arnebia euchroma(Royle.) Johnst.【Method】The proper combination of growth regulator was screened by using the stem-tip of Arnebia euchroma(Royle) as a explant,proliferate and root-age.【Result】The results showed that the optimal germination medium was MS+TDZ 1.0 mg/L,whose germination rate was 100% and the bud multiplication medium was MS+TDZ 1.0 mg/L with 13.50 folds and successive healthy seedling medium was MS+NAA 0.3 mg/L+6-BA 0.5 mg/L,the best rooting medium was MS+NAA 0.1 mg/L,the rate of rooting was 84%.Where the consentration of 6-BA decreased by 0.05,the rate of vitrification could be largely decreased and when cultured in this medium,the rooting rate could increased to 95.32%.【Conclusion】Stem tip is the best explant of Arnebia euchroma(Royle.) Johnst rapid propagation

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