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Position: Home > Articles > Assay Study on VC in Guava Fruits by High Performance Liquid Chromatography FOOD SCIENCE 2007,28 (4) 292-295

番石榴中VC的高效液相色谱分析

作  者:
刘胜辉;臧小平;魏长宾
单  位:
中国热带农科院南亚热带作物研究所
关键词:
番石榴;VC;高效液相色谱
摘  要:
采用高效液相色谱法,以0.1%的磷酸作为流动相,采用三种不同的提取液(3%的偏磷酸、3%的偏磷酸+8%乙酸、0.1%的草酸)对番石榴不同的果实部位的VC(L-抗坏血酸)含量进行了测定,结果表明:3%的偏磷酸+8%乙酸作为浸提液效果最好,对于三次取样的结果相对标准偏差最低(RSD=1.6%),回收率最高为98.5%;3%的偏磷酸提取效果次之;0.1%的草酸溶液提取效果最差,RSD=28.4%,回收率也很低(72.5%)。此外,不同部位的番石榴果肉VC含量也不一致,以靠近果皮的果肉含量最高,其次为果心,中部果肉含量最低。
译  名:
Assay Study on VC in Guava Fruits by High Performance Liquid Chromatography
作  者:
LIU Sheng-hui ZANG Xiao-ping WI Chang-bin (South Subtropical Crops Research Institute of Scutas,Chinese Acadenny of Tropic Agricultural Sciences,Zhanjiang 524091,China)
关键词:
guava fruits;;vitamin C;;HPLC(high performance liquid chromatography)
摘  要:
Determination of vitamin C(V/C)in guava fruits by HPLC with 0.1% orthophosphoric acid as the mobile phase was studied.Three different solutions were used to extract the VC.The method with 3% metaphosphoric acid-8% acetic acid showed high mean recoveries(98.5 %),while the LC-extraction method with 0.1% oxalic acid was proved to be unacceptable in some cases with mean recoveries as low as 72.5%.The good precision(RSD=1.6%)is achieved with the metaphosphoric acid-acetic acid LC--extraction method for determining VC in guava fruits with recovery as high as 98.5%,as a perfect routine analysis method.The content of VC in the fruit varied with different parts.The pulp close with the pericarp contained the higest content as 220.4mg/100g,while the middle part the lowest with only 85.78mg/100g.

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