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Position: Home > Articles > Study on a Capture ELISA with McAb for Detection of Antibodies against AEV Progress in Veterinary Medicine 2002,23 (1) 72-75

单抗捕捉ELISA检测AEV抗体方法的研究

作  者:
杨建民;秦卓明;苏敬良;赵继勋;郝永新
单  位:
山东家禽研究所;宁夏畜牧兽医研究所;中国农业大学实验动物所
关键词:
禽脑脊髓炎;单克隆抗体;单抗捕捉ELISA;抗体检测
摘  要:
本研究初步建立了检测AEV抗体的单抗捕捉ELISA 方法。AEV腹水单抗(腹水效价为1 ∶1×106)最佳稀释度为1∶200,AEV抗原工作浓度为16.08 μg/mL,血清稀释度为1∶1 00, 兔抗鸡酶标抗体工作浓度为1∶500。经阻断试验、交叉试验、重复试验,以及与进口试剂 盒 进行平行检测40份血清,总符合率为92.5%,表明该方法特异性强、重复性好、敏感性高, 是一种有应用前景的方法。
译  名:
Study on a Capture ELISA with McAb for Detection of Antibodies against AEV
作  者:
YANG Jian-min1, QIN Zhuo-ming2,SU Jing-liang1, ZHAO Ji-xun1 ,HAO Yong-xin3 (1.China Agricultural University, Beijing haidian ,100094,China; 2.Shan dong poul try research institute, Jinan ,250100,China;3.Ningxia Veterinary Medicine and animal husbandry
关键词:
AEV;monoclonal antibody;capture ELISA;detection of antibodies
摘  要:
: The procedure of capture ELISA for detection of AEV an tibodies were e stablished with the monoclonal antibodies against AEV, and the best concentratio ns of the monoclonal antibodies, the AEV and HRP-Rabbit antichichen were 1/200 , 16.08 μg/mL, and 1/500 respectively.The good stability of capture ELISA were v er ified by inhibited tests, cross tests , reproducible tests,parallel tests with E LISA kit from America (IDEXX).

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