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Position: Home > Articles > The Preparation and Application of Agar Gel Preciptin Antigen for Avian Encephalomyelitis Progress in Veterinary Medicine 2001,22 (2) 61-62

禽脑脊髓炎琼扩抗原的研制和应用

作  者:
张淑霞;杨增岐;李金钧;赵余放;李东成;薛登民
单  位:
西北农林科技大学禽病研究室;西北农林科技大学;农科城正源畜牧兽医研究所
关键词:
禽脑脊髓炎;琼扩抗原;特异性
摘  要:
将禽脑脊髓炎病毒 (AEV)陕西分离株接种于 6日龄鸡胚卵黄囊 ,1 6日龄时收取活胚脑、胃肠道和胰腺 ,匀浆灭活后制成琼扩抗原 ,该抗原与 AE标准阳性血清在 36 h内出现特异性的沉淀线 ,而与 ND、EDS- 76、AI、IB阳性血清及 SPF鸡血清不出现沉淀线 ,用其检测 6 0份血清 ,48份呈 AE阳性 ,与用标准抗原检测的结果一致
译  名:
The Preparation and Application of Agar Gel Preciptin Antigen for Avian Encephalomyelitis
作  者:
ZHANG Shu xia 1, YANG Zeng qi 1, LI Jin jun *, ZHAO Yu fang 2, LI Dong cheng 2, XUE Deng min 2 (1.Avian Disease Research Group of Northweastern Sci Tech University, of Agriculture and Forestry, Yangling Shaanxi 712100,China;2.Institute
摘  要:
The Avian Encephalomyelitis Virus (AEV) strain separated from Shaanxi province was injected into chicken embryo yolksac of 6 days.The brains gastrointestinal tracts and pancreas of the embryos were collected at 16 days. After homogenation and inactivation,they were used as the Agar Gel preciptin(AGP) and produced specific precipitate line with the AE possitive standard serum.But ND,EDS 76,AI and IB possitive serum and the serum from SPF chicken had no precipitate line with AE possitive serum. The antigen was used to test 60 serum samples.48 of them were AE possitive ,just the same as that from the standard AE antigen. Key wrods: AE; AGP antigen; specificity

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