摘 要：
猪IL-15与人IL-15具有较高的相似率,为进一步研究其生物活性,本研究将去除信号肽的猪IL-15克隆至原核表达载体pET-30a,并成功获得其重组蛋白。将纯化的重组蛋白用于多克隆抗体的制备,并利用琼脂扩散方法、ELISA方法、Western blotting试验及免疫荧光试验对其抗体效价、特异性、灵敏度等生物活性进行了检测。结果表明,该抗体具有较高的抗体效价和很好的特异性,可以作为IL-15特异性抗体用于今后的试验研究。
译 名：
Preparation and Biological Activity Analysis of the Polyclone Antibody to Porcine IL-15
作 者：
ZOU Yu-fei,WANG Heng,YUAN Li-guo,LI Shou-jun(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China)
关键词：
porcine IL-15;polyclonal antibody;biological activity
摘 要：
Porcine IL-15 has a high similarity to human IL-15.Porcine IL-15 without signal peptide was cloned into prokaryotic expression vector pET-30a,the recombinant plasmid was transformed into E.coli BL21,and induced by IPTG.Recombinant protein was aquired successfully,then the purified protein was used to prepare polyclone antibody.Agarose diffusion assay,ELISA,Western blotting assy and immunofluorescence were used to detect the titer,specificity and sensitvity of the antibody.The results revealed that the polyclone antibody prepared in this study had a high titer and good specificity,and it could be used as the specific antibody to porcine IL-15 for the future research.
