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Position: Home > Articles > Control Technique for Embryo Morphological After-ripening of Acanthopanax sessiliforus Guizhou Agricultural Sciences 2014 (12) 84-87+91

无梗五加种胚形态后熟调控技术

作  者:
郭志欣;顾地周;沈红梅;闫中雪;袁浩;徐鹏佳
单  位:
通化师范学院生命科学学院
关键词:
无梗五加;形态后熟;调控技术;发育
摘  要:
为探索无梗五加种子形态后熟的影响因素,以无梗五加种子为供试材料,通过多因子交叉重复试验对无梗五加种子形态后熟的主要影响因素进行探讨。结果表明:无梗五加种子形态成熟的最优条件是将种子用2.10~2.30mg/L GA3和3.40~3.50mg/L 2,4-D混合溶液浸泡60h,将种子与河砂(1∶4)混合,进行150~160d的变温处理,变温幅度和时间为白天温度(24±2)℃,处理12h,夜间温度(15±2)℃,处理12h,心形胚发生率99.7%,子叶胚发生率98.9%,播种后发芽率97.5%。
译  名:
Control Technique for Embryo Morphological After-ripening of Acanthopanax sessiliforus
作  者:
GUO Zhixin;GU Dizhou;SHEN Hongmei;YAN Zhongxue;YUAN Hao;XUE Pengjia;College of Life Science,Tonghua Normal University;
关键词:
Acanthopanax sessiliforus;;morphological after-ripening;;control technique;;development
摘  要:
A.sessiliforus seeds were tested to study main factors of influencing morphological afterripening of Acanthopanax sessiliforus seeds by a multiple-factor cross repeat test.The results showed that the optimum conditions for morphological after-ripening of A.sessiliforus seeds include that seeds are soaked with the mixed solution of 2.10~2.30mg/L GA3 and 3.40~3.50mg/L 2,4-D for 60 h,and seeds mixed with river sand(1∶4)are treated by changing temperature(24±2℃for 12 hat daytime and 15±2℃for 12 hat night)for 150~160d,and the occurrence rate of the heart-shaped embryo,cytoledon embryo and germination of A.sessiliforus seeds treated by the optimum conditions is 99.7%,98.9% and97.5% separately.

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