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Position: Home > Articles > Preparation and characterization of polyclonal antibody to Diethylstibestrol China Animal Health Inspection 2006,23 (5) 23-25

己烯雌酚多克隆抗体的制备和鉴定

作  者：
古艳丽;郑增忍;刘文字;陈德坤;路平;刘爽;李葳;曲志娜
单  位：
农业部动物检疫所;华南农业大学兽医学院;西北农林科技大学动物科技学院
关键词：
己烯雌酚;免疫原;包被原;多克隆抗体
摘  要：
用混合酸酐法合成的BSA-DES免疫兔子,制备抗DES的特异性抗体,分别用混合酸酐法和碳二亚胺法合成的OVA-DES作为包被原检测抗体;用混合酸酐法合成的OVA-DES和碳二亚胺法合成的OVA-DES免疫小白鼠,制备抗DES的特异性抗体,分别用混合酸酐法合成的BSA-DES和碳二亚胺法合成的BSA-DES作为包被原检测抗体。检测结果表明:三种免疫原免疫动物,都产生了DES的特异性抗体,并建立了间接阻断ELISA法。这为残留DES酶免疫检测试剂盒的制备奠定了基础。
译  名：
Preparation and characterization of polyclonal antibody to Diethylstibestrol
作  者：
GU Yan-li1) QU Zhi-na2)* CHEN De-kun1) ZHENG Zeng-ren2) LU Ping2) LIU Shuang2) LI Wei2) (1. Animal Husbandry and Veterinary Science, Northwestern Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi ,712100, China; 2.Amimal Quarantine Institute , Ministry of Agricultur , Qingdao, 266032, China)
关键词：
Diethylstibestrol; immunogen; coating antigen; polyclonal antibody
摘  要：
To prepare the anti-DES antibody, rabbits were immunized with BSA-DES which was synthesized in mixed anhydride and isobutylchlorofomate, and OVA-DES which were synthesized respectively by mixed anhydride and Isobutylchorofomate and carbodiimide method were used coating antigens to identify antibody in ELISA; Mice were immunized by OVA-DES which respectively were synthesized by mixed anhydride and Isobutylchorofomate and carbodiimide method, and BSA-DES which were synthesized respectively by mixed anhydride and Isobutylchorofomate and carbodiimide method were coating antigens to identify antibody in ELISA; The results show that anti-DES polyclonal antibody were produced by rabbits and mice, and indirect inhibition ELISA method was established. It's very important to develop DES residual immunoassay reagent kit.

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