当前位置: 首页 > 文章 > 马鹿茸不同部位IGF-1含量的比较分析 经济动物学报 2005,9 (4) 201-203
Position: Home > Articles > Comparative Analysis of IGF-1 Contents in Different Segments of Cervus elaphus linnaeus Journal of Economic Animal 2005,9 (4) 201-203

马鹿茸不同部位IGF-1含量的比较分析

作  者:
郝林琳;刘松财;任晓慧;戴建威;王伟;张永亮
单  位:
吉林大学农学部畜牧兽医学院
关键词:
马鹿茸;多肽;胰岛素样生长因子1;比较分析
摘  要:
采用醋酸溶液和乙醇提取了6支马鹿茸的主杠尖部、中部、根部3个部位的多肽,进行了IGF-1的SDS-PAGE和Western blot分析,采用RIA法测定了马鹿茸酸粗提物及醇提多肽的IGF-1含量。结果表明,酸粗提物中IGF-1存在游离和与结合蛋白结合两种形式,醇提物中只存在分子量为7.5 ku的游离IGF-1。马鹿茸不同部位酸粗提物和醇提物之间IGF-1含量经RIA法检测表明,主杠尖部、中部、根部3个部位IGF-1含量差异显著,其中尖部IGF-1含量分别比中部和根部高43.10%(P<0.01)、284.83%(P<0.01),中部比根部高168.93%(P<0.01);经醇提后,各部位之间IGF-1含量差异显著,其中尖部IGF-1含量分别比中部和根部高42.33%(P<0.05)、164.04%(P<0.01),中部比根部高85.51%(P<0.05)。该试验为马鹿茸的分段利用提供了内在质量上的依据。
译  名:
Comparative Analysis of IGF-1 Contents in Different Segments of Cervus elaphus linnaeus
作  者:
HAO Lin-lin~1,LIU Song-cai~1,REN Xiao-hui~(1,2),DAI Jian-wei~1,WANG Wei~1,ZHANG Yong-liang~1 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.College of Aquaculture,Hebei Agricultural University,Qinhuangdao 066003,China)
关键词:
Cervus elaphus linnaeus;polypeptide;IGF-1;comparative analysis
摘  要:
In this study,acid extract and alcohol extract of six cervus elaphus linnaeus were obtained.Insulin-like growth factor 1(IGF-1)was identified by SDS-PAGE and Western blot.IGF-1 contents in different segments(top、middle & basis,three segments)were detected with RIA.The results of SDS-PAGE and Western blot showed that IGF-1 was extracted with acid and alcohol.IGF-1 contents of the acid extract in top was higher 43.10%(P<0.01)and 284.83%(P<0.01)than in middle and basis respectively,was higher 42.33%(P<0.05)and 164.04%(P<0.01) for alcohol extract polypeptide.IGF-1 contents of the acid extract in middle was higher 168.93%(P<0.01) than in basis,was higher 85.51%(P<0.05) for alcohol extract polypeptide.This test offered an internal quality index in subsection utilization of cervus elaphus linnaeus.

相似文章

计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊