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当前位置: 首页 > 文章 > 鲜马鹿茸不同部位多肽的提取及含量比较 吉林农业大学学报 2007,29 (4) 28-30+33
Position: Home > Articles > Extraction and Comparative Analysis of Polypeptides Contents of Different Segments in Cervus elaphus Linnaeus Journal of Jilin Agricultural University 2007,29 (4) 28-30+33

鲜马鹿茸不同部位多肽的提取及含量比较

作  者：
郝林琳;刘松财;张明军;程淑琴;吕铁钢;张永亮
单  位：
承德职业学院;吉林大学畜牧兽医学院
关键词：
马鹿茸;多肽;胰岛素样生长因子-1;表皮生长因子;神经生长因子
摘  要：
采用醋酸溶液和乙醇提取了6支鲜二杠马鹿茸的主干顶部(未骨化部分)、中部(部分骨化)、根部(大部分骨化)3个部位的多肽,进行了多肤的SDS-PAGE分析和胰岛素样生长因子-1(IGF-1)、表皮生长因子(EGF)、神经生长因子(NGF)的Western-blotting分析。采用考马斯亮蓝G-250法进行了马鹿茸不同部位总多肽定量,RIA法测定了IGF-1含量,ELISA法比较了不同部位EGF、NGF的OD值。结果:SDS-PAGE表明鹿茸酸醇提取多肽为一系列分子量低于17kD的混合物,Western-botting证明其含有IGF-1、EGF、NGF等生长因子。定量分析表明:马鹿茸顶部、中部、根部总多肽、IGF-1及醇提多肽中IGF-1含量均差异显著(P<0.05),EGF、NGF ELISA检测OD值差异显著(P<0.05),均从顶部到根部递减。
译  名：
Extraction and Comparative Analysis of Polypeptides Contents of Different Segments in Cervus elaphus Linnaeus
作  者：
HAO Lin-lin~1 LIU Song-cai~1 ZHANG Ming-jun~1 CHENG Shu-qin~2 L(?) Tie-gang~1 (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China; 2.Chengde Vocational College,Chengde 067000,China; 3.College of Animal Science,South China Agricultrual University,Guangzhou 510642,China)
关键词：
velvet antler;;polypeptides;;insulin-like growth factor 1;;epidermal growth factor;;nerve growth factor
摘  要：
We extracted the active components of different segments (top,middle & basis) of six fresh velvet anflers-polypeptides with acetic acid solution(pH 3.5)and alcohol and analyzed by SDS-PAGE and Western blotting with anti-human IGF-1、EGF、NGF as the first antibodies.The total polypeptides and IGF-1 contents in different segments of velvet antler were detected with Coomassie brilliaat blue G-250 and RIA techniques.EGF and NGF OD values were compared with ELISA.Results:The results of SDS -PAGE indicated that deer velvet antler polypeptides were a mix whose molecular were lower than 17 kD,and Western blotting revealed that IGF-1,EGF and NGF were extracted with acid and alcohol. Quantitative analysis showed that polypeptides and IGF-1 extracted with acid and alcohol,and IGF-1 extracted with acid in different segments of velvet antler were different significantly(P<0.05).The OD value difference of EGF and NGF contents between different segments of velvet antler showed by ELISA is also significant(P<0.05),all the data revealed that they are highly concentrated in the top of velvet antler(P<0.05).This test offered an internal quality index in subsection utilization of Cervus elaphus linnaeus.

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