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当前位置: 首页 > 文章 > A-FABP和H-FABP基因聚合基因型对中国环颈雉肌内脂肪含量的遗传效应分析 中国家禽 2014,36 (18) 6-9+13
Position: Home > Articles > Genetic Effect of Pyramiding Genotypes of A-FABP and H-FABP Gene on Intramusular Fat Content of Chinese Ring-necked Pheasant China Poultry 2014,36 (18) 6-9+13

A-FABP和H-FABP基因聚合基因型对中国环颈雉肌内脂肪含量的遗传效应分析

作  者：
吴琼;杨颖;荣敏;邢秀梅;杨福合
单  位：
中国农业科学院特产研究所;吉林省省部共建特种经济动物分子生物学国家重点实验室
关键词：
中国环颈雉;A-FABP;H-FABP;肌内脂肪
摘  要：
研究旨在探讨A-FABP和H-FABP基因聚合基因型对中国环颈雉肌内脂肪(IMF)含量的遗传效应。利用PCR-SSCP方法检测中国环颈雉A-FABP和H-FABP基因多态性,并对多态位点不同基因型及聚合基因型与中国环颈雉的胸肌和腿肌IMF含量进行关联性分析。结果显示,A-FABP基因和H-FABP基因分别检测到突变位点(C51T和T337C),产生3种基因型,关联性分析表明2个位点与中国环颈雉胸肌IMF含量显著相关(P<0.05),AA型和CC型为有利单基因型。两种有利单基因型聚合AACC基因型个体胸肌IMF含量显著高于其他基因型聚合个体(P<0.05)。A-FABP和H-FABP基因两两基因型合并,AACC基因型个体胸肌IMF含量最高。
译  名：
Genetic Effect of Pyramiding Genotypes of A-FABP and H-FABP Gene on Intramusular Fat Content of Chinese Ring-necked Pheasant
作  者：
WU Qiong;YANG Ying;RONG Min;XING Xiumei;YANG Fuhe;State Key Laboratory of Special Economic Animal Molecular Biology,Institute of Special Economic Animals and Plants,CAAS;
关键词：
Chinese ring-necked pheasant;;A-FABP;;H-FABP;;intramuscular fat
摘  要：
This study was conducted to study the genetic effect of A-FABP and H-FABP genes polymorphism and pyramiding genotypes with intramuscular fat(IMF)content of Chinese ring-necked pheasant. PCR-SSCP method was used to identify the polymorphism in A-FABP gene and H-FABP gene. Associations between polymorphism sites as well as pyramiding genotypes and IMF contents in breast and leg muscles were tested by least aquare analysis. Two polymorphism sites (C51T of A-FABP and T337 C of H-FABP) were indentified,which had three genotypes. The association analysis showed that polymorphism sites of A-FABP gene and H-FABP gene had significant effects on IMF content of breast muscle (P<0.05). The AA and CC were two advantageous genotypes. IMF content of breast muscle of pyramiding genotype AACC was higher than that of other pyramiding genotypes (P<0.05).The pyramiding genotype AACC had the highest IMF contents in muscle.

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