中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 海兰褐蛋鸡卵巢与输卵管FSHR及LHR基因定量的研究 黑龙江八一农垦大学学报 2015 (2) 27-31+45
Position: Home > Articles > Genetic Quantitative Research of FSHR and LHR in Ovary and Oviduct of Hy-line Brown Laying Hens Journal of Heilongjiang Bayi Agricultural University 2015 (2) 27-31+45

海兰褐蛋鸡卵巢与输卵管FSHR及LHR基因定量的研究

作  者：
何晶;耿仁德;计红;马莉;张虹亮;孔凡志;程晓旭;杨焕民
单  位：
黑龙江省农垦总局建三江分局勤得力农场第六管理区;黑龙江省农垦哈尔滨管理局动物卫生监督所;黑龙江八一农垦大学动物科技学院
关键词：
蛋鸡;促卵泡激素受体;促黄体生成素受体;基因表达量;荧光定量
摘  要：
为了研究鸡卵巢、输卵管子宫部、输卵管漏斗部FSHR及LHR基因表达水平差异,实验从海兰褐蛋鸡的子宫部、卵巢、漏斗部中提取总RNA,利用RT-PCR技术获得FSHR、LHR目的基因,并应用实时荧光定量PCR进行定量检测。结果显示卵巢组织FSHR m RNA表达水平显著高于子宫部与漏斗部组织(P<0.01),子宫部与漏斗部组织FSHR m RNA表达水平没有显著性差异;子宫部组织LHR m RNA表达水平极显著高于其他两组(P<0.01),但卵巢、漏斗部组织LHR m RNA表达水平没有显著性差异。实验通过研究鸡卵巢、输卵管子宫部、输卵管漏斗部FSHR及LHR基因表达水平差异,研究为今后研究禽类生殖生理提供基础数据和科学依据。
译  名：
Genetic Quantitative Research of FSHR and LHR in Ovary and Oviduct of Hy-line Brown Laying Hens
作  者：
He Jing;Geng Rende;Ji Hong;Ma Li;Zhang Hongliang;Kong Fanzhi;Cheng Xiaoxu;Yang Huanmin;Institute of Animal Health Supervision,Harbin Urban Administration of Land Reclamation Bureau in Heilongjiang Province;The Sixth District of Qindeli Farm of Jiansanjiang Land Reclamation Bureau,Heilongjiang Land Reclamation Bureau;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;
关键词：
hens;;FSHR;;LHR;;gene expression level;;fluorescence quantitative
摘  要：
In order to study the expression level of FSHR and LHR in ovary,the tubal uterine department and fallopian tube funnel department,ovary,tubal uterine department and fallopian tube funnel department were obtained from Hy-line brown laying hens,and the total RNA was extracted. FSHR and LHR target genes were amplificatied by using q RT-PCR,and the amount of FSHR and LHR were detected by fluorescent quantitative PCR. The results showed that FSHR m RNA expression level in ovarian tissue was extremely significantly higher than in the tubal uterine department and fallopian tube funnel department( P<0.01),and FSHR m RNA expression level in the tubal uterine department and fallopian tube funnel department had no significant difference. LHR m RNA expression level in tubal uterine department was extremely significantly higher than in ovary and fallopian tube funnel department( P<0.01),and LHR m RNA expression level in ovary and fallopian tube funnel department had no significant difference. The study illustrated the expression of FSHR and LHR in ovary,tubal uterine department and fallopian tube funnel department,which provided the foundation for the study on reproductive physiology of poultry.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46