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当前位置: 首页 > 文章 > 高效液相色谱-光电二极管阵列检测器法测定野山楂中两组分的含量 食品科学 2006,27 (11) 438-440
Position: Home > Articles > Determination of Two Components in Crataegus cuneata Sieb.et Zucc.by HPLC-PAD FOOD SCIENCE 2006,27 (11) 438-440

高效液相色谱-光电二极管阵列检测器法测定野山楂中两组分的含量

作  者：
邹盛勤;陈武
单  位：
宜春学院生物工程研究所江西省天然药物活性成分研究重点实验室
关键词：
野山楂;乌索酸;齐墩果酸;光电二极管阵列检测器;高效液相色谱;含量测定
摘  要：
建立高效液相色谱法测定了野山楂中乌索酸和齐墩果酸的含量,同时采用光电二极管阵列检测器检测两组分的紫外光谱。色谱柱为NucleosilC18柱(4.6mm×250mm,5μm),流动相为甲醇-水(87:13,V/V),流速0.8ml/min,检测波长210nm,柱温26℃。乌索酸在0.1244～2.488μg范围内线性关系良好(r=0.9996),平均回收率为98.1%。齐墩果酸在0.1192～2.384μg范围内线性关系良好(r=0.9999),平均回收率为101.2%。方法简便、准确、重现性好、线性范围宽,适用于测定野山楂中乌索酸和齐墩果酸的含量。
译  名：
Determination of Two Components in Crataegus cuneata Sieb.et Zucc.by HPLC-PAD
作  者：
ZOU Sheng-qin,CHEN Wu (Bioengineering Research Institute,Yichun University,Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines,Yichun 336000,China)
关键词：
Crataegus cuneata Sieb.et Zucc;ursolic acid;oleanolic acid;photodiode array detector;HPLC; content determination
摘  要：
Adetermination method of ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc. by reversed-phase high performance liquid chromatography was established and at the time UV spectrum of two components was detected by means of photodiode array detector(PAD). The chromatographic column,Nucleosil C18 (4.6mm×300mm,5μm),methanol- water (87: 13,V/V) mobile phase with 0.8ml/min flow rate,the detected wavelength (210nm),and the column temperature(26℃) were adopted. The calibration curve of ursolic acid was linear under the content of 0.1244～2.488μg,the correlation coefficient was over 0.9996.The average recovery of ursolic acid was 98.1%. The calibration curve of oleanolic acid was linear under the content of 0.1192～2.384μg,the correlation coefficient was over 0.9999. The average recovery of oleanolic acid was 101.2%. The method is simple,accurate,reproducible and has wider linear range. The method can be used to determine ursolic acid and oleanolic acid in Crataegus cuneata Sieb. et Zucc.

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