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Position: Home > Articles > Effects of Heparin Dosage and Sperm Capacitation Time on Ovine Sperm Capacitation and Fertilization in vitro Chinese Agricultural Science Bulletin 2010,26 (21) 21-24

肝素浓度和作用时间对绵羊精子体外获能和体外受精的影响

作  者:
刘烈琴;罗桂河;孟丹娜;薛君忠;郭勇;倪和民
单  位:
北京农学院动物科学技术系;北京盛世富民清真食品有限责任公司
关键词:
绵羊精子;体外获能;肝素;咖啡因;体外受精
摘  要:
通过研究添加不同的浓度肝素(0、5、10、20、50μg/mL)及不同作用时间(0、30、45、60、90、120min)对绵羊精子体外获能、活力、存活时间和相应体外受精的影响,为改善绵羊精子体外获能效果及研究获能的机理提供一定的参考。在获能液中添加10μg/mL肝素作用45min时,其获能率最高,顶体反应率最低(40.92%和16.59%)(P<0.05),存活时间18h,能够提高受精卵的囊胚发育;单独使用咖啡因或与10μg/mL肝素联合使用,其获能效果均不如单独使用10μg/mL肝素。说明绵羊精子获能时肝素的适宜浓度是10μg/mL,作用时间是45min,咖啡因无协同肝素促进获能的作用。
译  名:
Effects of Heparin Dosage and Sperm Capacitation Time on Ovine Sperm Capacitation and Fertilization in vitro
作  者:
Liu Lieqin 1,Luo Guihe 2,Meng Danna 2,Xue Junzhong 1,Guo Yong 1,Ni Hemin 1 (1 Department of Animal Science & Technology,Beijing University of Agriculture,Beijng 102206;2 Beijing Shengshi Fumin Islamic Food Co.Ltd,Beijing 102200)
关键词:
ovine sperm;capacitation in vitro;heparin;caffeine;fertilization in vitro
摘  要:
In order to find out some modified methods of ovine sperm capacitation in vitro,the effects of their sperm capacitation,motility,enduring durations and fertilization after respectively adding with 0,5,10,20,50 μg/mL heparin at different time (0,30,45,60,90,120 min),were compared and analyzed.The results showed that ovine sperm capacitation rate was highest (40.92%),acrosome reaction was lowest (16.59%),enduring durations were longest (18 h),and the blastula rate was highest,when adding with 10 μg/mL heparin in the capacitation medium for 45 min.The effects of capacitation when adding with caffeine or caffeine and 10 μg/mL heparin,were not as good as the effects adding with 10 μg/mL heparin alone.It can be recommended that it is applicable for improving ovine capacitation in vitro system with 10 μg/mL heparin for 45 min,caffeine don ' t cooperate with heparin to improve the rate of capacitation.

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