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Position: Home > Articles > The Study of Ovine Sperm Acrosome Reaction induced by Zone Pellucide Chinese Agricultural Science Bulletin 2011,27 (23) 29-31

透明带诱导绵羊精子顶体反应的研究

作  者:
刘烈琴;顾申;周卫宾;杨飞鸽;张宏;郭勇;刘云海;倪和民
单  位:
北京农学院动物科学技术系
关键词:
绵羊精子;获能;顶体反应;透明带;钙离子载体
摘  要:
为探讨绵羊透明带对其精子顶体反应的诱导作用,试验分为3组:①不经获能处理的精子与透明带共孵育,②精子经获能处理后与透明带共孵育,③不经获能处理的精子与透明带共孵育,然后添加IA23187,来观察透明带对精子顶体反应的诱导效果。结果显示获能与否并不影响透明带对精子顶体反应的诱导效果,添加IA23187后,顶体反应升高,但差异不显著(P>0.05)。说明透明带可诱导绵羊精子(无论获能与否)发生顶体反应,IA23187不能提高透明带对顶体反应的诱导作用。
译  名:
The Study of Ovine Sperm Acrosome Reaction induced by Zone Pellucide
作  者:
Liu Lieqin,Gu Shen,Zhou Weibin,Yang Feige,Zhang Hong,Guo Yong,Liu Yunhai,Ni Hemin (Department of Animal Science & Technology,Beijing University of Agriculture,Beijng 102206)
关键词:
ovine sperm;capacitation;acrosome reaction;zone pellucide;calcium ionophore
摘  要:
To find out the effects of ovine sperm acrosome reaction induced by zone pellucid,three experiments were designed:first,co-incubation non-capacited sperm with zone pellucid;second,co-incubation capacitation sperm with zone pellucid;third,adding with calcium ionophore A23187 after co-incubation non-capacited sperm with zone pellucid.The results showed that the rates of acrosome reaction induced by zone pellucid are not significant difference whether the spermatozoa were capacited,IA23187 don ' t cooperate with zone pellucide to improve the rate of acrosome reaction.

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