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当前位置: 首页 > 文章 > 仔猪心肌细胞分离及培养方法研究 中国畜牧兽医 2010,37 (3) 52-54
Position: Home > Articles > Study of Methods on Myocardial Cells Isolation and Culture in Piglets China Animal Husbandry & Veterinary Medicine 2010,37 (3) 52-54

仔猪心肌细胞分离及培养方法研究

作  者：
吴旭颖;郭亮
单  位：
天津农学院动物科学系
关键词：
心肌细胞;细胞培养;仔猪
摘  要：
为探讨仔猪心肌细胞的分离及培养方法,采用胶原酶Ⅳ型和胰酶同时消化心肌组织,用10%胎牛血清终止消化,并用100目细胞筛过滤后,1200 r/min离心沉淀细胞,再加入培养液重悬细胞。采用差速贴壁法纯化心肌细胞后置CO2培养箱孵育。结果显示,未贴壁时心肌细胞呈圆形,培养12~24 h心肌细胞开始贴壁生长,细胞伸出伪足呈梭形、多角形,3~4 d后形成细胞簇,5~7 d后细胞汇合成片。因此,同时使用胶原酶Ⅳ型和胰酶消化仔猪心肌可简便、快速地获得高活力的心肌细胞。
译  名：
Study of Methods on Myocardial Cells Isolation and Culture in Piglets
作  者：
WU Xu-ying,GUO Liang(Department of Animal Science,Tianjin Agricultural College,Tianjin 300384,China)
关键词：
cardiocyte;cells culture;piglet
摘  要：
To found an easy way to isolate and culture myocardial cells of piglets,the hearts were digested by collagenase type Ⅳ and trypsin.The digestion was stopped by adding 10% FBS.Cardiocytes were filtered by 100 item of cells sieve and precipitated by centrifugation,and then added the medium to cardiocytes.Cells were purificated to reduce fibroblast content by adhere bottle and then incubated in the CO2 incubator.The results showed that the cells were round before adhesion;12 to 24 hours later,the cells of fusiform and polygon began to adhere and stretch out the parapodium;3 to 4 days later,cell clusters formed;5 to 7 days later,the cells grew together flakily.Large cardiocytes with well morphology and high livability could be convenient and quickly obtained by digesting of collagenase type Ⅳ and trypsin.

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