中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 猫杯状病毒不同分离株免疫原性的比较 吉林农业大学学报 2015,37 (5) 612-616
Position: Home > Articles > Comparison of Immunogenicity of Different Feline Calicivirus Isolates Journal of Jilin Agricultural University 2015,37 (5) 612-616

猫杯状病毒不同分离株免疫原性的比较

作  者：
王一鸣;赵艳丽;赵立峰;应瑛;王开;胡桂学;杨松涛
单  位：
吉林农业大学动物科学技术学院;军事医学科学院军事兽医研究所
关键词：
猫杯状病毒;分离株;免疫原性;疫苗
摘  要：
为确定猫杯状病毒(Feline calicivirus,FCV)分离株JL-1、JL-2、JL-3、JL-4、SH免疫原性强弱,将各株灭活后分别制备油乳剂疫苗。将6~12周龄健康非免疫家猫随机分为免疫组和对照组。免疫组皮下接种相应毒株制成的灭活苗,对照组接种等量F81细胞培养液。于接种后不同时间点采集猫血清,应用ELISA方法检测免疫猫Ig G抗体、IL-2、IL-4和IFN-γ含量,评价各株诱导的体液免疫水平;采用MTT法测定淋巴细胞增殖,评价各株诱导的细胞免疫水平。结果表明:各株均能诱导试验猫产生免疫应答,其诱导体液免疫顺序依次为JL-2>JL-1>JL-4>JL-3>SH,诱导细胞免疫顺序依次为JL-4>JL-2>JL-1>SH>JL-3,初步确定各分离株免疫原性顺序为JL-2>JL-4>JL-1>JL-3>SH。
译  名：
Comparison of Immunogenicity of Different Feline Calicivirus Isolates
作  者：
WANG Yiming;ZHAO Yanli;ZHAO Lifeng;YING Ying;WANG Kai;HU Guixue;YANG Songtao;College of Animal Science and Technology ,Jilin Agricultural University;Institute of Military Veterinary Medicine,Academy of Military Medical Sciences;
关键词：
Feline calicivirus;;isolate;;immunogenicity;;vaccine
摘  要：
To identify immunogenicity of Feline calicivirus( FCV),five isolates were inactivated and then mixed with freund's adjuvant. Approximately 6-12 week-old healthy unvaccinated kittens were randomly allocated to vaccinated( V) and control( C) groups. Kittens of group V were vaccinated with five isolates respectively twice at a 2-week interval via the subcutaneous route,while group C received equal amount of F81 cell culture. The sera specific of all isolates were collected at different time. The content of Ig G,IL-2,IL-4 and IFN-γ was measured by ELISA Kit to evaluate humoral immunity level and peripheral lymphocyte proliferation measured by MTT assay to evaluate cell immunity level induced by five isolates. The results showed that all isolates could induce immune response of vaccinated kittens with the order of humoral immunity level: JL- 2 > JL- 1 > JL- 4 > JL- 3 > SH,and cell immunity level: JL- 4 > JL- 2 > JL- 1 > SH > JL- 3. These data demonstrate preliminarily that the immunogenicity order of five isolates was: JL- 2 > JL- 4 > JL- 1 > JL- 3 >SH.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46