当前位置: 首页 > 文章 > 枯草芽孢杆菌挥发产物的杀线虫活性评价及成分鉴定(英文) 植物病理学报 2009,39 (3) 304-309
Position: Home > Articles > Evaluation and Identification of the Potential Nema-ticidal Volatiles Produced by Bacillus subtilis Acta Phytopathologica Sinica 2009,39 (3) 304-309

枯草芽孢杆菌挥发产物的杀线虫活性评价及成分鉴定(英文)

作  者:
刘玮玮;季静;王超;慕卫;刘峰
单  位:
中国石油化工股份有限公司;山东农业大学园艺科学与工程学院;山东农业大学植物保护学院
关键词:
枯草芽孢杆菌;挥发性有机化合物;杀线虫活性;固相微萃取-气相色谱/质谱联用技术;生物防治
摘  要:
寄生线虫Ditylenchus destructor是许多作物和蔬菜的重要病原物。本研究进行的密封皿内测定结果表明,由枯草芽孢杆菌BL02和G8产生的挥发物能够强烈地抑制D.destructor(24h杀虫活性均达100%)。经挥发物处理后,大部分线虫在1~12h内活动逐渐减弱,24h后完全停止活动。经固相微萃取并经气相色谱-质谱联用技术进一步鉴定,挥发物包括烷烃、醇、酯、酮、酸、胺、肟、酚及杂环类。本研究为微生物活性挥发物的开发和利用提供了有价值的参考并预示了细菌挥发物在未来植物线虫病害生物防治方面的应用潜力。
译  名:
Evaluation and Identification of the Potential Nema-ticidal Volatiles Produced by Bacillus subtilis
作  者:
LIU Wei-wei1,JI Jing1,WANG Chao2,MU Wei1,LIU Feng1(1College of Plant Protection,Shandong Agricultural University,Tai'an 271018,China;2College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an 271018,China)
关键词:
Bacillus subtilis;volatile organic compounds(VOCs);nematicidal activity(NA);SPME-GC/MS;biocontrol
摘  要:
Plant-parasitic nematode Ditylenchus destructor is one of the most serious pathogens of crops and vegetables.In this study,volatiles produced by two Bacillus subtilis strains BL02 and G8 both displayed 100% nematicidal activity(NA) against D.destructor in sealed dishes.When exposed to these nematicidal volatiles,the nematodes gradually reduced movement within 1-12 h and stopped completely after 24 h.The effective volatiles were extracted using solid-phase micro-extraction(SPME) and further identified by gas chromatography-mass spectrometry(GC-MS) technique.The detected volatile organic compounds(VOCs) included alkyls,alcohols,esters,ketones,acid,amine,oxime,phenols and heterocyclic compounds.This study provided useful information for the exploitation of microbial bioactive volatiles and was the first report about the potential application of bacterial volatiles in biocontrol of plant-parasitic nematode.

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