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Position: Home > Articles > Enzymatic Preparation of Antioxidant Peptides from Channel Catfish (Ictalurus punctatus) Skin Gelatin FOOD SCIENCE 2010,31 (19) 254-258

鮰鱼皮明胶抗氧化肽的制备工艺研究

作  者：
王运改;林琳;李明辉;翁世兵;叶应旺;姜绍通;陆剑锋
单  位：
合肥工业大学生物与食品工程学院
关键词：
鮰鱼;酶解;活性肽;羟自由基
摘  要：
通过测定鮰鱼皮明胶蛋白酶解物对Fenton体系产生的羟自由基的清除效果,筛选得出胰蛋白酶和胃蛋白酶酶解物具有较高的清除羟自由基(·OH)的活性;用正交试验L9(34)对胰蛋白酶的水解条件进行优化,确定最佳的水解条件为温度40℃、pH7.5、酶与底物质量浓度比(E/S)3.5%、底物质量浓度2.5g/100mL、酶解时间3h。此外,本研究还采用胰蛋白酶和胃蛋白酶进行了复合酶解试验,确定复合酶解的最佳水解条件为先用胰蛋白酶酶解3h,然后用胃蛋白酶酶解3h,此时得到的酶解液自由基清除率最高,达到47.38%。
译  名：
Enzymatic Preparation of Antioxidant Peptides from Channel Catfish (Ictalurus punctatus) Skin Gelatin
作  者：
WANG Yun-gai,LIN Lin,LI Ming-hui,WENG Shi-bing,YE Ying-wang,JIANG Shao-tong,LU Jian-feng (College of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China)
关键词：
channel catfish (Ictalurus punctatus);enzymatic hydrolysis;bioactive peptides;hydroxyl free radical
摘  要：
Five enzyme preparations were solely used to hydrolyze channel catfish (Ictalurus punctatus) skin gelatin and pepsin and trypsin were screened for further studies in view of higher hydroxyl free radical scavenging activity of the hydrolysates. Optimization of the conditions for trypsin hydrolysis was carried out using orthogonal array design L9(34) and the optimal hydrolysis conditions were determined as follows:temperature 40 ℃; pH 7.5; enzyme/substrate ratio (E/S) 3.5%; substrate concentration 2.5 g/100mL; and hydrolysis duration 3 h. In addition,pepsin and trypsin were used together for the two-step hydrolysis of channel catfish gelatin in different orders of addition or one-step hydrolysis,namely adding the two enzymes simultaneously. Initial trypsin hydrolysis for 3 h followed by another 3 h of pepsin hydrolysis resulted in the generation of peptides with the highest hydroxyl free radical scavenging rate,up to 47.38%.

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